Differentiation of liver function cells from human and mouse stem cells

Nowadays, stem cells have become a popular topic not only in scientific activities but also in all areas of life. The reason stem cells increasingly attracts the attention of the society because they have wide applicability, from biomedical field, serving the treatment of many dangerous diseases such as: diabetes, paralysis due to spinal cord injury, heart failure due to damage to the heart muscle, some cancers and genetic diseases,… to applications in rehabilitation and beauty treatment.

With the purpose of assessing the ability to differentiate liver function cells generation and localization of stem cells in liver disease stem cell transplant models, the Institute of Biotechnology has implemented the project “Research on differentiating liver function cells from human and mouse stem cells", code: VAST.DL.03 / 16-17, implementation time from June 2016 to December 2019 by TS. Nguyen Van Hanh is the project’s leader. 

The results obtained during the research process provided more scientific information on isolation capacity, stem cells multiplication, differentiate research, implant on a diseased mouse model, related issues and the potential of stem cells in cell differentiation and transplantation. 

The project has obtained scientific information related to the ability to acquire human stem cells and mouse bone marrow stem cells with specific markers and the ability to maintain stable culture under in vitro conditions. The obtained stem cells source has been evaluated for its full characteristic stem cells potential, cultured and maintained under in vitro conditions, eligible for basic research and other application studies in the future. The thesis has created cells with functional indicators of liver cells from human mesenchymal stem cells and mouse bone marrow stem cells when processing stem cells with different differentiation formulas (Photo 1).

Post-differentiated cells show positive liver function indicator (in blue for the nucleus, green for HNF4a proteins for a specific indicator of liver cells)

The obtained source of liver function cells has been assessed to be capable of developing to become a possible source of cells to use in research on liver disease models and further development to help in screening new substances and medicines. The project has also created a model to evaluate the ability of locating stem cells, liver function cells differentiated from stem cells on the liver disease model by attaching stem cells to luminescent diamond nanoparticles (Photo 2).

Human stem cells attached with luminescent diamond nanoparticles (red dots are cell-attached diamond nanoparticles)

This study presented for the first time information regarding stem cell transplants, differentiated liver function cells tagged with diamond nanoparticles in liver disease model mouse. The results showed the potential in stem cell applications in transplantation, as well as using mouse models to monitor cell function in many different diseases. The study also showed for the first time the efficiency of differentiated liver function cell transplants in a mouse model of liver disease (Photo 3). 

Human stem cells attach luminescent diamond nanoparticles locally in mouse liver tissue

After 42 months of implementation, from June 2016 to December 2019, the topic was evaluated as excellent by the Council. The results of the project open up a potential research direction on using different stem cell sources in biomedical research, as well as to model the screening of bioactive substances and apply the model to evaluate the localization capacity of stem cells. 

Translated by Phuong Ha
Link to Vietnamese version


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