Controlling coffee cicadas with biological products containing the nematode Steinernema phuquocense n. sp. S-PQ16
Because pesticides pollute the environment and affect human health, many countries around the world, including Vietnam, are gradually turning to use biological measures in plant protection. One of the methods is to use parasitic nematodes that cause insect diseases (Entomopathogenic Nematodes – EPN for short).
In Vietnam, the Institute of Ecology and Biological Resources is the first unit to research and use EPN nematodes to control pests in agriculture. Over the years, after testing in the laboratory and in the field in some localities with good results, the Institute has begun to transfer production technology to a number of units.
Nematode probiotics are very suitable for the control of soil pests, where 85% of insect species have at least one stage of existence and development in the soil. However, the ability to kill pests living on leaves is still limited.
For example, in order to kill grapevine worms in Ninh Thuan, the nematodes must be combined with sugar molasses to create moisture and adhesion for the nematodes to survive and kill pests. So far, no nematode probiotics have been produced that have the ability to effectively control cicadas that damage coffee plants. Therefore, there is still a need for effective and environmentally friendly preparations and methods that can effectively control cicadas that damage coffee trees.
“Method of controlling coffee cicadas with biological products containing the nematode Steinernema phuquocense n. sp. S-PQ16” mentions the method of controlling coffee cicadas with a biological product containing the nematode Steinernema phuquocense n. sp. S-PQ16 isolated and kept by the Institute of Ecology and Biological Resources.
The invention proposed a biological product for the control of coffee cicadas by a new nematode strain designated SPQ16 and identified as a new nematode with scientific name Steinernema phuquocense n. sp. The login codes for new species-specific gene segments at the International GenBank (GenBank) are KX405172 (ITS-rDNA region) and KX405173 (28S region). Biological products containing the nematode S-PQ16 have composition per 1000mL of neutral clean water containing 20,000,000 infective larvae (IJ), 1mL of Triton X-100 lubricating additive and 1mL of formalin antibacterial agent.
This product has the ability to kill coffee cicadas and some other pests. It is possible to apply invivo technology using large wax moth larvae (Galleria mellonella) or house crickets (Acheta domestic), or invitro technology using chicken offal artificial environment to produce probiotic nematode S-PQ16 to serve biological control at different scales.
The invention also proposed a method for the control of coffee cicadas consisting of steps for the application of a biological product containing the nematode S-PQ16 on cicada-infested coffee plantations. The test spraying of a biological product containing S-PQ16 nematode in cicada-infected soil to determine the control effect showed that S-PQ16 nematode not only has the ability to kill cicadas but also has the ability to regenerate in the soil environment.
Translated by Tuyet Nhung
Link to Vietnamese version