| Main results | - Theoretical results: * Obtained the list of fisf, mollusc and crustacea related to TTX, mollusc related to DA, crustacea related to STX, and reef fish related to CTX with the information on taxomomy, photos and specimens. * Developed 12 practical prtocols in extraction and analysis of TTX, STX, DA and CTX in seafood for safety (included evaluation information). - Applied results: Studied results have been applied to determine quatitatively TTX, STX, DA and CTX in seafood for food safety in domestic and exported products. Novelty and actuality and scientific meaningfulness of the results: As the first time in Vietnam, the practical protocols on extraction and analysis toxins (TTX, STX, DA and CTX) in marine animals have been developed and applied in VAST Keylab “Environment and food safety in central of Viet Nam” in Institute of Oceanography. Further research on marine toxins and seafood safety in Viet Nam can be carried out in large scale. |
| Products of the project | - Scientific papers in referred journals (list):
1. Ha Viet Dao, Aya Uesugi, Hajime Uchida, Ryuichi Watanabe, Ryoji Matsushima, Zhen Fei Lim, Steffiana J Jipanin, Ky Xuan Pham, Minh-Thu Phan, Chui Pin Leaw, Po Teen Lim, Toshiyuki Suzuki (2021). Identification of Fish Species and Toxins Implicated in a Snapper Food Poisoning Event in Sabah, Malaysia, 2017. Toxins 2021, 13, 657. https://doi.org/10.3390/toxins13090657. (ISSN: 2072-6651; SCIE; IF: 4.2; Q1).
2. Ha Viet Dao, Hy Ho Khanh Le, Thao Thi Thu Le, Ky Xuan Pham, Minh Quang Bui & Leo Lai Chan (2022). Ciguatoxin in moray eels raising the risk for seafood safety in Viet Nam. Fisheries Science 88, 821-830. https://doi.org/10.1007/s12562-022-01638-6. (e.ISSN:1444-2906; Print ISSN:0919-9268; IF: 1.9; Q2)
3. Dao Viet Ha, Le Ho Khanh Hy, Pham Xuan Ky, Bui Quang Nghi, Nguyen Phuong Anh, Phan Bao Vy, Doan Thi Thiet (2023). Frequent occurrence of tetrodotoxin in the marine gastropod Nassarius glans causing a food poisoning in Khanh Hoa province, Vietnam in 2020. Vietnam Journal of Marine Science and Technology 23(2), 203–208. https://doi.org/10.15625/1859-3097/18080. (ISSN 1859-3097; e-ISSN 2815-5904).
4. Pham Thi Mien, Jutta Wiese, Dao Viet Ha (2023). Metabolites from the soft coral -associated bacterium Micrococcus sp. strain A-2-28. Vietnam Journal of Marine Science and Technology 23(2), 181–188. https://doi.org/10.15625/1859-3097/18414 (ISSN 1859-3097; e-ISSN 2815-5904).
5. Pham Thi Mien, Dao Viet Ha, Cornelis Verduyn (2023). Nutritional effects on biomass and metabolic products by Aurantiochytrium sp. Vietnam Journal of Science, Technology and Engineering, Part C. 65(4). 94-100. DOI: 10.31276/VJSTE.65(4).94-100.
6. Dao Viet Ha, Le Ho Khanh Hy, Pham Xuan Ky, Nguyen Ngoc Tung, Hajime Uchida (2021). Identification of Ciguatoxin-1b using LC/MS-MS with Selected Ion Monitoring (SIM) technoque in Vietnam. The Proceeding on The 7th analytica Vietnam Conference. Ha Noi. ISBN: 978-604-369-618-9.
- Technological products (describe in details: technical characteristics, place):
+ List of marine toxic species in Viet Nam including photo and specimens.
* Scientific names, Vietnamese names and pictures of three marine fish species containing tetrodotoxin in Vietnam.
* Scientific names, Vietnamese names and pictures of three marine fish species containing ciguatoxin in Vietnam.
* Scientific names, Vietnamese names and pictures of three marine molusks species containing tetrodotoxin in Vietnam.
* Scientific names, Vietnamese names and pictures of one marine molusks species containing domoic acid in Vietnam.
* Scientific names, Vietnamese names and pictures of two marine crustacea species containing tetrodotoxin in Vietnam.
* Scientific names, Vietnamese names and pictures of one marine crustacea species containing saxitoxin in Vietnam.
+ 06 practical protocols for quantitative analysis of natural toxins from marine fauna in Vietnam:
* Quantitative analysis of tetrodotoxins in marine fish in Vietnam using HPLC-MS LCMS 8040 Shimadzu;
* Quantitative analysis of tetrodotoxins in marine molluscs in Vietnam using HPLC-FD Shimadzu 20AD;
* Quantitative analysis of tetrodotoxins in marine crustaceans in Vietnam using HPLC-FD Shimadzu 20AD;
* Quantitative analysis of saxitoxin in marine crustaceans in Vietnam using HPLC-FD Shimadzu 20AD and post-column reaction system;
* Quantitative analysis of domoic acid in marine bivalve in Vietnam using HPLC-UV Shimadzu 20 AC;
* Quantitative analysis of ciguatoxin in reef fish in Vietnam using LCMS 8040 Shimadzu;
+ 06 practical protocols for extracting natural toxins from marine fauna in Vietnam:
* Extracting tetrodotoxin from some puffer fish species in Vietnam
* Extracting tetrodotoxin from marine molluscs in Vietnam
* Extracting tetrodotoxin from marine crustaceans in Vietnam
* Extracting domoic acid from marine bivalve in Vietnam
* Extracting saxitoxin from marine crustaceans in Vietnam
* Extracting ciguatoxin from coral reef fish in Vietnam
+ General scientific report.
- Other products: The project has successfully supported 02 masters, of which 01 master has received the degree:
+ Do Thi Thai Nguyen. Establishing a practical protocol for quantitative analysis of residued chloramphenicol in some animal products using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in Vietnam. Supervisors: Assoc. Prof. Dao Viet Ha, Dr. Bui Quang Minh. According to decision No. 275/QD-HVKHCN dated March 19, 2021. Recognized graduation and awarded the master's degree according to decision No. 873/QD-HVKHCN dated July 7, 2023.
Vo Thi Doan Trang. Establishing a practical protocol for the analysis of tetrodotoxin in marine snails in Vietnam using LC-MS/MS. Supervisor: Assoc. Prof. Dao Viet Ha. According to decision No. 1434/QD-HVKHCN dated September 21, 2022. Decision No. 1286/QD-HVKHCN dated November 1, 2023, on the establishment of the Master's Thesis Evaluation Council. Defensed on November 22, 2023. |
