The main goal of the project were screening and searching for sources of biologically active sulfated polysaccharide compounds from seaweeds and marine invertebrates; searching for strains of marine bacteria and marine fungi capable of producing enzymes to metabolize marine polysaccharides.

- For the polysaccharides extracted from seaweed, we researched the fucoidan from 2 Vietnamese brown seaweeds of Sargassum oligocystum and Sargassum serratum. In particular, the chemical components of fucoidan from S. serratum seaweed have been determined, including total carbohydrates, sulfate, uronic acid, and simple sugar components. In addition, combined with 1H-NMR, MS, and EIM-MS/MS spectrum measurement results, the structure of this fucoidan was also clearly defined, which has a linear backbone built up of alternating (1-3) and (1-4) fucose. Besides, fucoidan from S. serratum was also proven to have the ability to inhibit the growth of liver cancer line HepG2. At the tested sample concentration of 100 mg, the size of the cancer line HepG2 decreased by 33,56% compared to the control sample.
- For the sulfated polysaccharides extracted from marine invertebrates, we researched GAGs from a Vietnamese sea cucumber, Holothuria leucospilota. The concentration of extracted GAGs was 4,66%. This GAG was fractioned into three fractions (F1, F2, and F3) by ion-exchange chromatography, and the chemical compositions of this GAG that included carbohydrate, sulfate, uronic acids, and monosaccharides compositions were determined, which combined with IR, 1H-NMR, and 13C-NMR IR spectrum measurement results to conclude that F1 and F2 were FCS type, and F3 was FS type. Also in this research, the anticoagulant activities of fraction F2 and F3 were tested, and the results showed that the activity that F2 possessed was even better than Heparin at evaluated tests of APTT, TT, and PT at two concentrations of 50 and 100 mg/mL.
- For the research of marine organisms-derived polysaccharides degraded enzymes, 58 bacterial isolates isolated from 16 marine samples, of which 12 and 46 isolates were isolated from samples of seaweeds and invertebrates, respectively, were screened. Among them, 26 isolates had the potential to produce polysaccharide-metabolizing enzymes. Then, six isolates capable of producing enzymes with the highest activity were identified as Bacillus velezensis 2105OB.23.12, Bacillus sp. 2105OB.6.1; Cobetia amphilecti 2105OB.4.2; Alteromonas sp. 2105OB.5.3, Alteromonas sp. 2105OB.25 và Vibrio sp. 2105OB.19.6.
- For the research of biological activities of marine fungi species, 61 marine fungi isolates isolated from 36 marine samples, of which 21 and 23 isolates were isolated from samples of seaweeds and invertebrates, respectively, were screened. All isolates possess antibacterial or antioxidant activity or both. Then, six isolates capable of producing biological compounds with the highest activity were identified as Penicillium sp. 2105OF.8.7, Aspergillus sp. 2105OF.24.3, Pleosporales sp. 2105OF.29.2, Talaromyces sp. 2105OF.31.2, Aspergillus terreus 2105OF.35.5 và Hypomontagnella monticulosa 2105OF.52.1..

- Determination of structural characteristics and data on anti-tumor of seaweed Sargassum seratum-derived fucoidan.
- Obtained glycosaminoglycans from a new species of sea cucumber that has never been studied before in Vietnam, Holothuria leucospilota, with the potential to possess good anticoagulant activity, and the research data has also been used for training a Master of Chemistry.