Project's information
| Project's title | Study of lipid composition and investigate biological activity of lipid classes from marine species collected during the 7th expedition aboard of research vessel “Academic Oparin” in the Vietnamese maritime zones |
| Project’s code | QTRU02.11/21-22 |
| Research hosting institution | Institute of Natural Products Chemistry |
| Coordinating unit, co-chair | Far East Branch of the Russian Academy of Sciences (FEB RAS) |
| Project leader’s name | Prof. Dr. Pham Quoc Long and Dr. Pavel Dmitrenok |
| Project duration | 01/04/2021 - 30/06/2024 |
| Project’s budget | 1,000 million VND |
| Classify | Excellent |
| Goal and objectives of the project | - Establish database of lipid, and investigating the biological activities of lipids of samples collected by the 7th research vessel Akademik Oparin in Vietnam’s oceans |
| Main results | - Research: • In the 7th research vessel Akademik Oparin in Vietnam’s oceans, a total of 64 samples were collected, selecting to research on 33 marine samples( registering 30 samples). The samples include 7 coral species, 7 mollusc species, 8 seaweed species, 6 echinoderm species and 5 sponge species. All samples are identified, classified, and stored. • Total lipid extraction was performed and the total lipid content of 33 samples was determined. Perform analysis and obtain all data on the composition and content of total lipids classes (registering 30 samples), composition and content of fatty acids of all 33 marine samples (registering 30 samples). Obtained results on the composition and content of phospholipid classes of 26/33 marine samples (registering 05 samples). • The determined phospholipid molecular species of the sea cucumber sample Thelenota ananas including 14 PE molecular species, 5 PC, 3 PS and 1 PI; ; and 11 molecular species PA of the sea urchin Tripneustes gratila. • After building and optimizing the three-dimensional structure of the research conpounds and identify the receptor target S. aureus Gyrase enzyme, molecular docking simulations were performed to predict binding affinity and clarify the binding mechanism between the polar ends of phospholipids and the targeted protein. The results show that the binding affinity of PI to the receptor target is significantly stronger than that of other polar heads including PS, PE, and PC. • 33 lipid extract samples were tested for activity, showing that: + The results of the cell growth inhibition activity assessment using the MTT method on two cancer cell lines, HepG2 (liver cancer) and MCF-7 (breast cancer), revealed that 4 out of 7 extracts from Coral sediment, 5 out of 8 extracts from Seaweed, 1 out of 6 extracts from Sea Cucumber, and 2 out of 5 extracts from Sea Urchin exhibited inhibitory activity against both liver and breast cancer cell lines. Soft Tissue extracts did not show any activity on the tested cell lines. + None of the 33 research samples demonstrated antioxidant activity on the DPPH system. + Among the Seaweed samples, 2/8 showed activity against 1 to 4 strains of microorganisms, and particularly, the red seaweed Amphiroa fragilissima exhibited activity against 4 strains of microorganisms at the initial test concentration of 200 μg/ml, with a minimum inhibitory concentration (MIC) ranging from 150 to 200 μg/ml. The remaining samples did not exhibit activity against the tested strains of microorganisms in the study. The obtained data will be the newly added information for the database on lipid profiles of marine organisms in Vietnam for the first time. - International Cooperation: With the support of equipments and modern analysis methods from Russian experts, the project has organized 01 trip for scientific exchange in the Laboratory of Comparative Biochemistry – Zhirmunsky Nationl Scientific Center of Marine Biology– FEB - RAS, collaborate to conduct researches to obtain results together. From the obtained results, the scientists from both side have published 01 international journal, 01 domestic journal - Training: contribute in training 01 Master (graduated) Full name: Đào Thị Hồng Nhung (2023) Thesis titles: Study of lipid composition and fatty acids in two sea cucumber species, Thelenota ananas and Holothuria edulis, collected from Bai Dong Island, Ha Long Bay, Vietnam. |
| Novelty and actuality and scientific meaningfulness of the results | The project conducted lipid research on 33 marine samples collected during the 6th Oparin survey vessel in Vietnam. The samples include 7 coral species, 7 mollusc species, 8 seaweed species, 6 echinoderm species and 5 sponge species. All samples are identified, classified, and stored. Total lipid extraction was performed and the total lipid content of 33 samples was determined. Perform analysis and obtain all data on the composition and content of total lipids classes, composition and content of fatty acids of all 33 marine samples. Obtained results on the composition and content of phospholipid classes of 26/33 marine samples. Identified 23 phospholipid molecular species of the sea cucumber sample Thelenota ananas including 14 PE molecular species, 5 PC, 3 PS and 1 PI. After building and optimizing the three-dimensional structure of the research conpounds and identify the receptor target S. aureus Gyrase enzyme, molecular docking simulations were performed to predict binding affinity and clarify the binding mechanism between the polar ends of phospholipids and the targeted protein. The results show that the binding affinity of PI to the receptor target is significantly stronger than that of other polar heads including PS, PE, and PC. 33 lipid extract samples were tested for activity, showing that 4/7 coral extract samples, 5/8 grassweed samples, 1/6 echinoderm samples and 2/5 sponge samples showed inhibitory activity against with both liver cancer and breast cancer cell lines, 2/8 Seaweed samples, 1/6 Echinoderm samples showed activity against 1 to 4 strains of microorganisms, especially the red seaweed sample Amphiroa fragilissima showed activity against 1 to 4 strains of microorganisms. Resistance to 4 microbial strains tested with initial sample testing concentration of 200 µg/ml and minimum inhibitory concentration (MIC) in the range of 150 to 200 µg/ml. |
| Products of the project | - Publications: |
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