Project's information

Project's title Studies on the chemical composition of some marine organisms and microorganisms collected from the 7th expedition aboard the R/V "AKADEMIC OPARIN" in Vietnam, to obtain bioactive compounds
Project’s code QTRU02.10/21-22
Research hosting institution Institute of Marine Biochemistry (IMBC)
Project leader’s name Assoc. Prof. Doan Thi Mai Huong
Project duration 01/04/2021 - 30/04/2024
Project’s budget 1000 million VND
Classify Excellent
Goal and objectives of the project

- Search for bioactive compounds from marine microorganisms collected in the 7th survey collection trip in the North Central region of Vietnam;
  - Enhance the cooperation with the Pacific Institute of Bioorganic Chemistry to contribute to training staffs.

Main results

- Participated in activities on the journey, collected sediment and marine samples in the North Central sea areas of the seventh survey trip by the marine research ship “Oparin Academik”.
- Collected 43 marine organisms and sediment samples at 23 different coordinates, from Bach Long Vy island - Hai Phong to Ly Son island - Quang Ngai province by the marine research ship "Oparin Academik", including: 8 species of molluscs, 11 species of seaweed, 8 species of sponge, 7 species of soft coral, 2 species of marine animals, 1 species of echinoderm and 6 sediment samples.
- Isolated 66 strains of microorganisms, screened 66 strains for biological activity. 
- Identified 6 bioactive microbial strains, including: G817 and G819 belonging to the species Salinispora arenicola; G222 relating to the genus Streptomyces; G824 is highly similar to Pseudonocardia carboxydivorans; OM01 and OM07 are closely related to strains of the species Penicillium malachiteum.
- Fermented in large quantities (50 kg/strain) of 2 bioactive strains: Penicillium sp. OM01 and OM07
- Isolated and determined the structures of 20 compounds from two bioactive strains of Penicililum OM01 and Penicililum OM07, including 1 new compound penicritin (OM01-1) and known compounds as follows: phenol A acid (OM01-2), topsentisteron D3 (OM01-3), ergosterol (OM01-4), (22E,24R)-24-methylcholesta-5,22-diene-3β,7β-diol (OM01-5), 5α,6α-epoxy-(22E,24R)-ergosta-8,22-diene-3β,7α-diol (OM01-6), 4-(2-hydroxyethyl)phenol (OM01-7), adenine (OM01-8), 2’-deoxythymidine (OM01-9), 2’-deoxyuridine (OM01-10), diketopiperazine dimer WIN 64821  (OM07-1), ergosterol peroxide (OM07-2), ergosterol (OM07-3), 3β,5α,9α-trihydroxyergosta-7,22-dien-6-one (OM07-4), 3,4-dihydroxy-6,7-dimethyl-quinolin-2-carboxylic (OM07-5),   norhaman (OM07-6),   dihydrocitrinin (OM07-7),  phenol A acid (OM07-8), adenosine (OM07-9) and 2’-deoxyadenosine (OM07-10).
- Carried out the antimicrobial and cytotoxicity activity tests of the isolated compounds
- The results of the project create the foundation to develop the cooperation between the two sides in the field of research and isolation of marine microorganism-derived bioactive compounds
- Trained 01 bachelor

Novelty and actuality and scientific meaningfulness of the results

- Isolated 66 strains of microorganisms and screened the biological activity (antimicrobial activity and KB cytotoxic activity) of the extracts of the isolated strains.
- Identified 6 bioactive microbial strains, including: G817 and G819 belonging to the species Salinispora arenicola; G222 relating to the genus Streptomyces; G824 is highly similar to Pseudonocardia carboxydivorans; OM01 and OM07 are closely related to strains of the species Penicillium malachiteum.
- Isolated and determined the structures of 18 pure compounds from Penicililum strains OM01 and OM07, including 1 new compound, penicritin (OM01-1). The biological activity results of selected compounds showed that 14 compounds exhibited the antimicrobial activity (the new compound penicritin (OM01-1) showed the antimicrobial activity against 2 Gram (+) bacteria with MIC values of 32 and 64 µg/mL). Compounds OM07-2 (ergosterol peroxide) and OM07-6 (norhaman) showed moderate cytotoxic activity against KB and Hep-G2 cancer cell lines.

Products of the project
Published articles
International articles:
- Van Cuong Pham, Thi Hue Nguyen, Thuy Linh Nguyen, Mai Anh Nguyen, Thi Quyen Vu, Thi Thu Huyen Vu, Thi Hong Minh Le, and Thi Mai Huong Doan ,2024, Antimicrobial activities of a new alkaloid from marine-derived fungus, Penicillium sp. OM01, Natural Product Communication (Accepted).
Domestic articles:
- Vu Thi Quyen, Le Thi Hong Minh, Nguyen Mai Anh, Pham Van Cuong, Vu Thi Thu Huyen1, Doan Thi Mai Huong, 2023, Isolation, identification of antimicrobial activity possessing marine actunomycetes, fungi from samples collected at the Bach Long Vy to Ly Son Island, Vietnam Journal of Biotechnology 21: 1-14.
- Phi Thi Dao, Nguyen Thuy Linh, Vu Van Nam, Vu Thi Quyen, Nguyen Mai Anh, Vu Thi Thu Huyen, Le Thi Hong Minh, Pham Van Cuong and Doan Thi Mai Huong, 2024, Antimicrobial secondary metabolites from a marine-derived fungus Penicillium sp. OM07, Journal of Marine Science and Technology, 24 (2), p.109-112.  
Images of project
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