Project's information
| Project's title | Biological evaluation of isolated compounds from Physalis alkekengi grown in Belarus and Vietnam |
| Project’s code | QTBY01.01/22-23 |
| Research hosting institution | Center for High Technology Development |
| Coordinating unit, co-chair | Belarus Basic Research Foundation |
| Project leader’s name | Associate Prof. Dr. Hoang Le Tuan Anh and Dr. Vladimir N. Reshetnikov |
| Project duration | 01/01/2022 - 31/12/2023 |
| Project’s budget | 200 million VND |
| Classify | Excellent |
| Goal and objectives of the project | - Isolate main components and compare the key active compounds of Physalis alkekengi grown in Belarus and Vietnam.
- Evaluate and screen several bioactivities of isolated compounds. |
| Main results | In Vietnam
Phytochemistry
- 07 compounds were isolated and structurally elucidated from Physalis alkekengi, including physalin D (PA1), physalin F (PA2), physalin G (PA3), physalin B (PA4), Withanolide J (PA5), withaperuvin C (PA6), and physaperuvin G (PA7). Besides, 08 compounds were isolated from Physalis peruviana in Vietnam and these compounds were identified as withaperuvin C (PP7), 28-hydroxywithaperuvin C (PP8) physaperuvin G (PP5) withaperuvin (PP6) perulactone 3-glucoside (PP2), Withanolide J (PP3), Physapruin A (PP4). In particular, compound PP1 was a new natural compound named as withaperuvin O.
- A comparison of phytochemical constituents of Physalis alkekengi in Belarus and Physalis alkekengi in Vietnam indicated that both species contained the same main active compounds: physalin D (PA1), physalin F (PA2), physalin G (PA3), physalin B (PA4), Withanolide J (PA5), withaperuvin C (PA6).
Bioactivities:
- The cytotoxicity of isolated compounds on A549, HeLa, and PANC-1 cell lines was examined. As a result, compounds PA2, PA3 và PA4 showed strong cytotoxic effect on A549 cells with IC50 values from (0.68 ± 0.05 to 0.95 ± 0.04 µM), (compared to positive control, etoposide (IC50 2.68 ± 0.89 µM). In addition, compound PP2 also strongly inhibits HeLa cells with an IC50 value of (0.23 ± 0.03 µM) [positive control, etoposide (IC50 3.29 ± 0.05 µM)]. On the HepG2, withaperuvin C (PP7), physaperuvin G (PP5), withaperuvin (PP6), Withanolide J (PP3), Physapruin A (PP4) showed significant activities. Furthermore, compound PP3 exhibited a strong inhibitory effect on NO production with IC50 values from (3.18 ± 0.22 µM) (compared to positive control, L-NMMA (IC50 7.72 ± 0.46 µM).
In Belarus.
- Comparative analysis of the content of main secondary metabolites in two Physalis species has shown that the content of bioactive substances in Physalis alkekengi is higher than the content of active substances in Physalis angulata. Specifically, flavonoids in the leaf tissue of P.alkekengi are 45.6% higher, hydrocinamic acids are 30.8% higher, and phenolics are 6.1% higher than those in P. angulata. The content of physalactone (one of the forms of vitasteroid with strong anti-cancer activity) in P.angulata leaves is 4.9% higher than that of P.alkekengi.
- Determining the optimal environmental conditions for in vitro leaf motor culture of two species P.alkekengi (Belarus) and P.angulata (Vietnam) corresponding to MS medium containing 3 mg/L BA: 2 mg/L 2,4-D and 1 mg/L BA : 3 mg/L 2,4-D.
- Determination of protein markers for plants belonging to the genus Physalis.
comparison of registered and achived results in table below
 - The results of the project have helped train and improve the professional capacity of young researchers of the Center, train students and postgraduates at universities or institutes, and improve the professional capacity of Belarusian researchers in the fields of Pharmaceutical Chemistry and Natural Compounds Chemistry. - Develop and expand cooperation in science and technology in many fields with the Central Botanical Garden, the Belarusian Institute of Science, and other Belarusian research agencies. |
| Novelty and actuality and scientific meaningfulness of the results | - For the first time, comparing the main active ingredients from Physalis alkekengi samples in Belarus and Physalis alkekengi as well as Physalis peruviana L. collected in Vietnam, the results showed that these species had the same main active ingredients, including physalin D (PA1 ), physalin F (PA2), physalin G (PA3), physalin B (PA4), Withanolide J (PA5), withaperuvin C (PA6). |
| Products of the project | - Publications: |
| Recommendations | - Identify and build in vivo anti-cancer and anti-inflammatory models for active pure substances, total extracts, and fractional extracts to confirm their activities. - Develop standards for medicinal materials of the Physalis genus. - Develop processes, transfer, and apply processes and basic standards in product production at enterprises. |
| Images of project |  |