Project's information

Project's title Femtosecond spectroscopy of ultrafast internal rotational dynamics of fluorescent molecular rotors
Project’s code QTBY01.06/22-23
Research hosting institution Institute of Physics
Coordinating unit, co-chair Basic Research Foundation of Belarus
Project leader’s name Prof. Dr. Pham Hong Minh and Prof.Dr.Sc. S. A. Tikhomirov
Project duration 01/01/2022 - 31/12/2023
Project’s budget 200 million VND
Classify Excellent
Goal and objectives of the project

- Study the kinetics of ultrafast processes for the intrinsic rotational dynamics of molecular rotors in the environment using femto-second spectroscopy. 
- Training science and technology staff, strengthening international cooperation and integration. Promoting the research capacity of Vietnam - Belarus Laboratory in optics and spectroscopy.

Main results

Successfully built a theoretical and experimental research model on the dependence of fluorescence intensity and anisotropy of the molecular complex of fluorescent dye SG labeled on double-stranded DNA (diDNA) formed in solution. Evaluate the influence of parameters such as labeled dye concentration, number of base pairs in the DNA chain, fluorescence quenching on anisotropic fluorescence intensity, fluorescence lifetime, and bond distance of dyes with DNA molecules as well as the interactive energy transfer of molecular rotors with biological structures. Thereby enhancing the effectiveness of fluorescent sensors based on this interaction. 
Measurements of the fluorescence polarization of SG molecules as a function of concentration revealed its decrease, which is related to Förster energy transfer. Using the developed model, which takes into account the homogeneous and rotational diffusion of the SG/dsDNA complex, this experimentally obtained dependence of the fluorescence anisotropy on the temperature and viscosity ratio average was interpreted and found a uniform rate in the FRET electron transfer of labeled SG molecules in dsDNA. 
In Vietnam, we have also expanded our research, searching for alternative structures for the dyes used as molecular rotors. Specifically, they successfully fabricated carbon quantum dots CQDs and nitrogen-doped CQDs using solution interaction plasma to enhance the luminescence properties of CQDs. The evaluated optical properties revealed changes in the fluorescence properties of N-CQDs compared to pristine CQDs. These preliminary research results will be the basis for the fabrication of doped CQDs and their applications in biological labeling and DNA structure analysis in the future.
During the two years of the project, we have had specific scientific exchanges between the two sides. The results of this research have contributed greatly to the training of young scientists in the field of quantum photonics and nano-photonics in Vietnam. It also promoted international publications, which is now a matter for the Vietnam Academy of Science and Technology.

 

Novelty and actuality and scientific meaningfulness of the results

The results of this research have contributed greatly to the training of young scientists in the field of quantum photonics and nano-photonics in Vietnam. It also promoted international publications, which is now a matter for the Vietnamese Academy of Science and Technology. 

Products of the project
- Scientific papers in referred journals (list):
1. S. A. Tikhomirov, A. P. Blokhin, V. A. Povedailo, A. S. Pilipovich, D. L. Yakovlev, F. Fan, V. V. Shmanai, P. H. Minh, and P. V. Duong, Resonance energy transfer between molecular rotors sybr green intercalated in DNA, Journal of Applied Spectroscopy, Vol. 90, No. 2, May, 2023, pp. 299-307, DOI 10.1007/s10812-023-01536-4
2. S.A. Tikhomirov, A.P. Blokhin, V.A. Povedailo, D.L. Yakovlev, Fan Fan, V. V. Shmanai, Pham Hong Minh, Pham Van Duong, Electronic energy transfer between sybr green molecular rotors intercalated in DNA, Advances in Optics, Photonics, Spectroscopy & Applications XII, 2022, ISBN: 978-604-357-120-2, pp. 137-141.
3. S.A. Tikhomirov , A.V. Povedailo, A. S. Pilipovich, T. F Raichenok, A. D. Shirokanov, D. L. Yakovlev, V.I. Stsiapura, Pham Hong Minh, Pham Van Duong, Twisted intramolecular charge transfer in benzothiazole-aniline molecular rotors, Advances in Optics, Photonics, Spectroscopy & Applications XII, 2022, ISBN: 978-604-357-120-2, pp. 142-146.
4. Pham Van Duong, Le Thi Thu Huong, Nguyen Tuan Anh, Do Minh Hieu, Nguyen Thi Khanh Van1, Nguyen Ngoc Anh, S. A. Tikhomirov, Nguyen Trong Tuyen, Nguyen Duc Khoang, Nguyen Thanh Cong, Pham Hong Minh, Nguyen Thanh Binh, Thai Doan Thanh, Optical properties of N‑doped carbon quantum dots fabricated by plasma-liquid interactions, Proceedings of The 8th Academic Conference on Natural Science for Young Scientists, Master and Ph.D Students from Asean Countries, 28-30 August 2023, Vinh City, Vietnam, 2023, pp. 682-686.
- Education: 01 Ph.D Student and 01 MSc. Student graduated.
Images of project
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