Project's information
| Project's title | Study on chemical constituents and cytotoxic activity of Sarcosperma kontumense |
| Project’s code | VAST04.10/20-21 |
| Research hosting institution | Institute of Marine Biochemistry (IMBC) |
| Project leader’s name | Nguyen Quoc Vuong |
| Project duration | 01/01/2020 - 30/06/2023 |
| Project’s budget | 600 million VND |
| Classify | Grade B |
| Goal and objectives of the project | Determination of chemical constituents of Sarcosperma kontumense and evaluation of their cytotoxic activity. |
| Main results | - Theoretical results:
+ From the stems and leaves of Sarcosperma kontumense, fiftheen compounds, including thirteen triterpenes and two flavonoids, were isolated and characterized.
* Thirteen triterpenes including:
Three triterpenes (1-3) possess hexacyclic skeleton with a 13,27-cyclopropane ring, which are very rare in nature. In which, there are two new compounds as sarcokontum A (1) và sarcokontum B (2) and a known compound as 2α,3α,19α,23-tetrahydroxy-13,17-cyclours-11-en-28-oic acid (3);
Nine known ursane-type triterpenes are corosolic acid (4), ursolic acid (5), pomolic acid (6), 1β,2α,3α,19α,23-pentahydroxyurs-11-en-28-oic acid (8), rotundic acid (9), euscaphic acid (10), jacoumaric acid (11), 3-O-trans-coumaroyltormetic acid and 3-O-cis-coumaroyltormetic acid (12);
A known olean skeleton triterpene is masilinic acid (7).
Compounds 1 – 5, 7-9, and 11-12 were isolated from the genus Sarcosperma for the first time.
* Two flavonoids are (-)-epi-catechin (14) and (+)-catechin (15).
+ Compounds 1-12 were evaluated to have none cytotoxic activity in concentrations 100 µM.
+ A laboratory-scale separation diagram for 10 clean compounds has been built. |
| Novelty and actuality and scientific meaningfulness of the results | - This is the first time a study on the chemical constituents of Sarcosperma kontumense was performed. The fiftheen compounds (1 - 15) were isolated and characterized, in which compounds 1 – 5, 7-9, and 11-12 were isolated from the genus Sarcosperma for the first time.
Especialy, from the stems and leaves of Sarcosperma kontumensem, two new triterpenes possessing hexacyclic skeleton with a 13,27-cyclopropane ring, which are very rare in nature as 2α,3β,19α,23-tetrahydroxy-13,17-cyclours-11-en-28-oic acid named sarcokontum A (1) and 2α,3β,19α,23-tetrahydroxy-13,17-cycloolean-11-en-28-oic acid named sarcokontum B (2) isolated and elucidated the structures. |
| Products of the project | Scientific papers in referred journals (list):
+ International publications: 01 SCI-E
Nguyen Quoc Vuong1*, Vu Van Chien1, Nguyen Thi Hue1, Pham Thi Hang1, Nguyen Hoang Nam1, Nguyen Le Tuan2, Pham Van Cuong1,3, Duong Thi Hai Yen1, Nguyen Xuan Nhiem1,3, Bui Huu Tai1,3, Phan Van Kiem1,3*; Sarcokontums A and B, Two New 13,27-Cyclo-Pentacyclic Triterpenes from the Stems and Leaves of Sarcosperma kontumense Gagnep. ex Aubrév., Natural Product Communications (2022) 17(11), 1–6.
+ Domestic publications: 01 Scopus
Nguyen Quoc Vuong1*, Vu Van Chien1, Nguyen Thi Hue1, Pham Thi Hang1, Nguyen Hoang Nam1, Nguyen Le Tuan2, Pham Van Cuong1,3, Duong Thi Hai Yen1, Nguyen Xuan Nhiem1,3, Bui Huu Tai1,3, Phan Van Kiem1,3*; Secondary metabolites from Sarcosperma kontumense, Vietnam Journal of Science and Technology 61 (6) (2023) 943-952.
- Patents (list):
- Technological products (described in details: technical characteristics, places/locations)):
+ The voucher specimen is deposited at the Saigon Botanical Museum by the Southern Institute of Ecology (VAST). Inernational code is SGN TNT1505202.
+ The fiftheen compounds (1-15) is deposited at the Department of Medicinal Chemistry Technology, Institute of Marine Biochemistry. |
| Recommendations | The ursane type triterpenes are the main compounds in the stem and leaves of Sarcosperma kontumense possessing many valuable biological activities, therefore further studies on their biological activities are necessary to thoroughly evaluate their effects in medicine. |
| Images of project |
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