Project's information
| Project's title | Genome sequencing and identification of genomic features related to antibiotic activity, cytotoxicity of endophytic actinomycetes from Litsea cubeba (Lour.) Pers. |
| Project’s code | ĐLTE00.03/21-22 |
| Research hosting institution | Institute of Biotechnology |
| Project leader’s name | Dr. Quach Ngoc Tung |
| Project duration | 01/01/2021 - 31/12/2022 |
| Project’s budget | 500 million VND |
| Classify | Grade B |
| Main results | - Theoretical results: The research has achieved the following results:
Of the 63 endophytic actinomycete strains from the plant Litsea cubeba (Lour.) Pers. in the VAST-Culture Collection of Microorganisms (VCCM), strain LCP02, LCP06 and LCP18 were the most potential strains with antibacterial activity against 8 tested pathogens. Besides, crude extract of the 3 strains at 100 μg/mL displayed strong inhibition against lung cancer A549, breast cancer MCF7 and hepatic cancer Hep-3B.
Based on morphological, biochemical characteristics and 16S rRNA sequence analysis, strain LCP02 was identified as S. cacaoi LCP02; strain LCP06 as S. albus LCP06; and strain LCP18 as S. variabilis LCP18.
In order to explore genomic features relating to antibacterial and anticancer activities, whole genomes of S. cacaoi LCP02, S. albus LCP06 and S. variabilis LCP18 were sequenced using Illumina platform. Three strains had large genome sizes varying between 7.39-8.44 Mb, of which functional genes comprised of 6830-7359 sequences.
Comparison of chemical and gene cluster analyses revealed a putative biosynthesis pathway of two plant-derived compounds such as daidzein and genistein in the genome of S. variabilis LCP18. UPLC-HR-MS/MS analysis resulted in 8 compounds from the crude extract of S. cacaoi LCP02, among which albonoursin and griseorhodin had the corresponding predicted gene clusters in LCP02 genome. Regarding S. albus LCP06, evaluation of genomic features and crude extract chemical compositions showed a gene cluster encoding the biosynthesis pathway of two isomers (3Z,6E)-1-N-methylalbonoursin and (3Z(E),6E(Z))-1-N-methylalbonoursin.
- Applied results: This project has contributed to the application of genome sequencing technology in research to further explore compounds with antibacterial and anticancer activity. The identified compounds from 03 strains possess the potential to be developed to be therapeutic compounds in pharmacology and medicine. |
| Products of the project | - Scientific papers in referred journals (list): |
| Images of project |  |