Vietnam Academy of Science and Technology

Project's information

Project's title	Study of chemical composition and evaluation of the inhibitory effect of α-glucosidase enzymes of Camellia petelotii and Camellia amplexicaulis
Project’s code	THTETN.02/21-23
Research hosting institution	Institute of Marine Biochemistry (IMBC)
Project leader’s name	Dr. Nguyen Thi Cuc
Project duration	01/06/2021 - 30/06/2023
Project’s budget	200 million VND
Classify	Grade B
Goal and objectives of the project	- Study of chemical composition of Camellia petelotii and Camellia amplexicaulis. - Evaluation of the inhibitory effect of α-glucosidase enzymes of compounds isolated from Camellia petelotii and Camellia amplexicaulis.
Main results	- The structure of 11 compounds (CP1-CP11) has been isolated and determined from the leaves of C. petelotii species. Among the isolated compounds were five new compounds: campeteloside A (CP1), campeteloside B (CP2), campeteloside C (CP3), campeteloside D (CP4) and campeteloside E (CP5). - The structure of 12 compounds (CA1-CA12) has been isolated and determined from the leaves of C. amplexicaulis species. Among the isolated compounds were four new compounds: amplexicoside A (CA1), amplexicoside B (CA2), amplexicoside C (CA3) and amplexicoside D (CA4). - Evaluated the inhibitory effect of the enzyme α-glucosidase of 11 compounds isolated from C. petelotii species was evaluated on an in vitro model. The results showed that four compounds CP1-CP3 and CP10 exhibited inhibitory activity of the enzyme α-glucosidase with IC50 values between 45.9 and 395.3 μM. - Evaluated the inhibitory effect of the enzyme α-glucosidase of 12 compounds isolated from C. amplexicaulis species on a in vitro model. The results showed that five compounds CA4, CA8, CA9, CA11 and CA12 had inhibitory activity of the enzyme α-glucosidase with IC50 values between 0.4 and 304.8 μM.
Novelty and actuality and scientific meaningfulness of the results	- Isolated and determined the structure of 5 new compounds (campeteloside A (CP1), campeteloside B (CP2), campeteloside C (CP3), campeteloside D (CP4) and campeteloside E (CP5)) from leaves of C. petelotii species. - Isolated and determined the structure of 4 new compounds (amplexicoside A (CA1), amplexicoside B (CA2), amplexicoside C (CA3) and amplexicoside D (CA4)) from leafy branches of C. amplexicaulis species. - This is the first report on the α-glucosidase inhibitory activity of all the isolated compounds.
Products of the project	- Scientific papers in referred journals (list): [1] Nguyen Thi Cuc, Ngo Anh Bang, Bui Huu Tai, Nguyen Xuan Nhiem, Pham Hai Yen, Duong Thi Hai Yen, Truong Thi Thu Hien, Le Thi Huyen, Phan Van Kiem. Five new oleanane triterpene saponins from Camellia petelotii and their alpha-glucosidase inhibitory activity. Chemistry & Biodiversity, (2023) n/a, e202300093. [2] Nguyen Thi Cuc, Duong Thi Dung, Ngo Anh Bang, Duong Thi Hai Yen, Nguyen Huy Hoang, Phan Van Kiem, Bui Huu Tai. Glycoside constituents of Camellia amplexicaulis and their α-glucosidase inhibitory activity. Natural Product Research, (2023), 1-9. - Technological products (deposited in the Institute of Marine Biochemistry): + Report on the isolation of 23 compounds from Camellia petelotii and Camellia amplexicaulis, and 23 isolated compounds. + Report on the structural determination of 23 compounds with their spectral data + Report on evaluation of α-glucosidase inhibitory activity of 23 isolated compounds.
Recommendations	Two compounds CA11 and cA12 exhibiting good activity inhibiting the enzyme α-glucosidase need further study to confirm and include in the list of guide compounds that inhibit the enzyme α-glucosidase.
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