Project's information
| Project's title | Study on effect of environmental factors, nutrients and reactors on biomass productivity and bioactivity of microalgal strain Chlorella sorokiniana |
| Project’s code | KHCBBI.01/19-21 |
| Research hosting institution | Institute of Chemistry |
| Project leader’s name | Dr. Tran Dang Thuan |
| Project duration | 01/01/2019 - 30/06/2023 |
| Project’s budget | 1500 million VND |
| Classify | Grade A |
| Goal and objectives of the project | - Study of the effects of environmental conditions (light intensity and types of light) and temperature on biomass yield and bioactivity (antimicrobial activity, anticancer activity) of the microalgal Chlorella sorokiniana; |
| Main results | - Theoretical results: + The effects of light intensity, temperature, NaCl and nitrate on biomass yield of C. sorokiniana TH01 was comprehensively investigated. The optimal conditions for maximal biomass production of C. sorokiniana TH01 were light intensity of 200 µmol/m2/s, temperature of 30 oC, aeration rate of 0,1 vvm, CO2 concentration of 5%, NaCl concentration of 20-25 g/L, NaNO3 concentration of 3 g/L, at which maximal biomass concentration achieved of 3.64 – 4.30 g/L; + Crude extracts of C. sorokiniana TH01 from different solvent extractions were investigated for anticancer activity on human cancer cell lines (KB, HepG2, MCF-7 và SK-LU-1). Data revealed that dichloromethane extract displayed the highest inhibition effect on the four human cancer cell lines among different solvents such as water, methanol, ethyl acetate, diethyl ether, dichloromethane and hexane with IC50 evaluated for KB, HepG2, MCF-7 and SK-LU-1 of 25,4, 18,2, 24,18 and 17,5 µg/L, respectively; + The effect of carbon source (glucose and sodium acetate) on biomass yield of C. sorokiniana TH01 under mixotrophic and heterotrophic cultivation was comprehensively investigated. For heterotrophic mode using glucose as a carbon source, the maximal biomass dry weight of 25 g/L was achieved under the optimal glucose concentration of 30 g/L. For mixotrophic mode, optimal conditions were glucose of 10 g/L or natri acetate of 8 g/L, light intensity of 150 µmol/m2/s, aeration rate of 0,1 vvm (2% CO2), achieving biomass concentration of 7.39 and 3.71 g/L, respectively; + Lutein was extracted and purified from C. sorokiniana TH01’s biomass, achieving purity of over 98%. Moreover, chemical structure was elucidated by UV-vis, NMR and MS techniques. Particularly, anticancer activity of the algal lutein was studied on human cancer cell lines including KB, HepG2, SK-LU-1, MCF-7). The obtained data revealed that IC50 of the algal lutein on KB, HepG2, MCF-7 và SK-LU-1 were 58.07 – 81.69 µg/mL; - Applied results: + Prototol for pilot-scale production of C. sorokiniana TH01 biomass was carried out in clumn photobioreactor (PBR), flat-panel photobioreactor (FPP) and open raceway (RW) via phototrophic and mixotrophic modes. Totally, over 3.9 kg C. sorokiniana TH01 biomass was produced for extraction and purification of bioactive compound (lutein); + Protocol for lutein extraction and purification from C. sorokiniana TH01 biomass achieving lutein purity of 98%. |
| Novelty and actuality and scientific meaningfulness of the results | + Prototol for pilot-scale production of C. sorokiniana TH01 biomass was carried out in clumn photobioreactor (PBR), flat-panel photobioreactor (FPP) and open raceway (RW) via phototrophic and mixotrophic modes; + Protocol of extraction and purification of lutein of over 98% purity from C. sorokiniana TH01; + Purified lutein with purity of over 98% (26,58 mg); + Anticancer activity of the algal lutein on four human cancer cell lines e.g., KB, HepG2, SK-LU-1, MCF-7. |
| Products of the project | - Scientific papers in referred journals (list): 03 ISI papers 1) Cam Van Thi Do, Nham Tuat Thi Nguyen, Mai Huong Thi Pham, Thanh Yen Thi Pham, Truong Giang Le, Dang Thuan Tran* (2022). Central composite design for simultaneously optimizing biomass and lutein production by a mixotrophic Chlorella sorokiniana TH01. Biochemical Engineering Journal, 177:108231. 2) Cuc T. Dinh, Cam Van T. Do, Thu Phuong T. Nguyen, Ngoc Hieu Nguyen, Truong Giang Le*, Thuan Dang Tran* (2022). Isolation, purification and cytotoxic evaluation of lutein from mixotrophically grown Chlorella sorokiniana TH01. Algal Research, 62:102632. 3) Cam Van T. Do, Cuc T. Dinh, Mai T. Dang, Thuan Dang Tran*, Truong Giang Le (2022). A novel flat-panel photobioreactor for simultaneous production of lutein and carbon sequestration by Chlorella sorokiniana TH01. Bioresource Technology, 345:126552. - Patents (list): 0 - Technological products (describe in details: technical characteristics, place): 02 technical reports - Other products (if applicable): 02 Master students |
| Research area | The valuable data achieved in this project is useful for internal and external research groups of VAST, carrying out the similar research direction. |
| Recommendations | I suggests Vietnam Academy of Science and Technology to continue supporting additonal fundings for our group to carry out following projects: |
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