Project's information

Project's title Research on the scientific basis for the use and conservation of Aristolochia species in Vietnam
Project’s code ĐLTE 00.09/21-22
Research hosting institution Vietnam National Museum of Nature
Project leader’s name Do Van Truong, PhD
Project duration 01/01/2021 - 31/12/2022
Project’s budget 600 million VND
Classify Grade A
Goal and objectives of the project
- Supplemental investigation of species compostition and assessemnt of conservation status of Aristolochia in Vietnam; 
- Studying chemical components of Essential oil of Aristolochia species in Vietnam; 
- Studying eligibility propagation of some Aristolochia species in Vietnam for conservation.
Main results
- Theoretical results:
31 Aristolochia species belonging to two subgenera: Siphisia (21 ssp) và Aristolochia (10 ssp), were recorded for Vietnam. Of these, four species were discovered and described as new to science; and two species were newly recorded for Vietnam. Furthermore, eighteen Aristolochia species in Vietnam were used as medicinal and ornamental plants. Based on the IUCN Red List Categories and Criteria, conservation status of twelve Aristolochia species were classified, ranging from Vunerable (VU) to Critical Endangered (CR).
86 herbarium specimens belonging to fourty-four collection numbers of twenty Aristolochia species were gathered and stored at VNMN.
The essential oils from the leaves and stems of eight Aristolochia species in Vietnam were successfully extracted. The results showed that the essential oils obtained from the leaves (0,182-1,147 ml/kg) are more than those of stems (0,091-0,231 ml/kg). Sixty-three chemical components of A. balansae were identified, which present 78.58% of the total composition of the leaves essential oil. This essential oil showed weak antimicrobial activities to most of the tested microbial strains in a concentration of 10%. While, thirty-four chemical components of A. xuanlienensis were identified, which present 52.98% of the total composition of the leaves essential oil. At a concentration of 1% of this essential oil showed no antimicrobial activities against any of the tested microbial strains.
The process of propagation by vegetative cuttings has been developed for Aristolochia species in Vietnam, and experimental propagation of five selected Aristolochia species has been developed. After three weeks, the budding rate reached ranging from 47.5% to 73.68%, in which A. tonkinensis achieved the highest budding rate (73.68%), followed by A. xuanlienensis (72.22%), A. neinhuisii (64.29%), A. vuquangensis (63.64%), and A. fangchi (47.5%). The process of vegetative cuttings budding usually lasts for a period of three-four weeks. Winter-spring period (from February to April) is the most suitable time for the propagation process, ensuring suitable temperature (14-200C) and air humidity (80-90%) for growth and development.
Publishing seven scientific papers, five of which were published in the SCI-E international journals (three in Phytotaxa; one in Annales Botanici Fennici; one in Acta Phytotaxonomica et Geobotanica), and two of which are published in the national journal: Vietnam Journal of Science, Technology and Engineering
Novelty and actuality and scientific meaningfulness of the results
- Updating and supplementing composition of Aristolochia species in Vietnam. In which, four species (A. vuquangensis, A. quangnamensis, A. luudamcuii, and A. thotteaeformis) are new to science, two species (A. versicolor and A. austroyunnanensis) are newly recorded for Vietnam, and two species (A. faviogonzalezii and A. versicolor) are newly recorded for the Flora of Laos.
- Firstly assessing conservation status of the whole Aristolochia species in Vietnam based on the IUCN Red List Categories and Criteria.
- Firstly identifying chemical components of the essential oils and testing antimicrobial activity of two selected Aristolochia species: A. balansae and A. xuanlienensis.
- Successfully developing the process of propagation by vegetative cuttings for Aristolochia species in Vietnam.


Products of the project
- Scientific papers in referred journals (list):
*) SCI-E international journals
1. Truong Van Do, Hung Viet Nguyen & Khuong Duy Le. 2021. Aristolochia vuquangensis (Aristolochiaceae), a new species from Central Vietnam. Phytotaxa 500 (1): 037–044.
2. Long Ke Phan, Stefan Wanke, Christoph Neinhuis & Truong Van Do. 2021. Aristolochia luudamcui (Aristolochiaceae), a new species from northern Vietnam. Phytotaxa 527 (1): 067–074. 
3. Truong Van Do, Hung Viet Lai, & Khuong Duy Le. 2021. Aristolochia quangnamensis (Aristolochiaceae), a new species from central Vietnam. Annales Botanici Fennici 58: 267–273.
4. Khuong Duy Le, Truong Van Do. 2022. Two new records of Aristolochia (Aristolochiaceae) for the Flora of Lao PDR. Acta Phytotaxonomica et Geobotanica 73 (2): 159–163.
*) National journals
5. Do Van Truong, Hoang Thi Thao. 2022. Aristolochia versicolor S.M.Hwang (Aristolochiaceae), a new record for the flora of Vietnam. Vietnam Journal of Science, Technology and Engineering 64(1): 49–52.
6. Lưu Đàm Ngọc Anh, Bùi Văn Hướng, Đỗ Văn Trường, Nguyễn Thị Kiều Oanh. 2022. Thành phần hóa học tinh dầu loài Aristolochia balansae Franch. và hoạt tính kháng vi sinh vật. Tạp chí Khoa học và Công nghệ Việt Nam (B), in press.
- Other products 
86 herbarium specimens belonging to 44 specimen number of 20 species are collected and stored at VNMN;
Essential oils of the eight studied species are extracted and stored at the laboratory of Centre for Environmental Bio-Geochemistry  of Vietnam National Museum of Nature.
Images of project