(1) Searching the genes of of HSP70 gene family in tomato (Solanum lycopersicum), and selecting potential genes with high adaptability to the environment;
Analysis of expression levels of genes HSP70 in tomato under adverse conditions;
(2) Determine the relationship between HSP70 proteins to the resistance to adverse conditions such as temperature, drought as well as the sensitivity of plants to adverse environmental conditions.

- Theoretical results: completed all the contents of the project, including:
The result of content 1:
- Identified the total of 25 genes in the HSP70 gene family in tomato plants. These genes carry specific sequences to the HSP70 gene family based on the database
- The HSP70 gene structural features and their distribution in the genome have been determined using bioinformatics methods, including the intron-exon structure, mass, size, and isoelectric point of the protein, the conserved sequences of the tomato HSP70 protein, proteins localization in the cell, including cytoplasm, endoplasmic reticulum, mitochondria, plastids, and nucleus.
- Phylogenetic analysis of HSP70 genes in tomato divided 25 HSP70 genes into 5 subfamilies A, B, C, D, E respectively based on their protein sequence, gene structure, and protein localization in the cell. The subfamily A contains 10 members with genes structure of 2 exons, and the proteins mainly distributed in the cytoplasm, followed by subfamilies B, C, D, E containing 5, 4, 4, and 2 genes, respectively. Their genes structure consisting of many exons and proteins localized on different organelles such as mitochondria, mitochondria, endoplasmic reticulum, and cell nucleus. The study also showed a closed relationship between the HSP70 gene family in tomato and potato, Arabidopsi and cleavage with the HSP70 gene family in rice.
- Collecting the data and analyzing the expression of the HSP70 gene family in diferent specific tissues under normal conditions showed that the HSP70 genes are expressed in most organs of the tomato plant imply that they can participate in many different physiological processes of tomato plants under normal conditions.
- Data collection and expression analysis of HSP70 gene family in tomato plants under adverse conditions of temperature, drought or salinity showed that HSP70 genes have different expression levels under adverse conditions of temperature, drought or salinity.
The result of content 2:
- RNA extraction, cDNA library synthesis and expression analysis of HSP70 gene family in tomato plants grown under adverse conditions of temperature, drought or salinity showed that HSP70 genes have different expression levels under different experimental conditions. There are 7 genes that responded to all heat, drought and salt treatments, as well as 11 members that responded to two types of stress, demonstrating that the extensive involvement of HSP70 genes in these external adverse conditions. Five HSP70 genes are recommended as potential candidates that play an important role in abiotic stress resistance.
The result of content 3:
- Transforming the HSP70-13 gene into Arabidopsis has proven the role of the gene, helping the plant to have better resistance, higher survival rate under adverse conditions of temperature, salinity and drought.

The preject results have explored and provided basic understanding of the number as well as the structural, evolutionary and expression characteristics of the HSP70 gene family under normal and adverse conditions in terms of temperature, salinity and drought.
The research results have also provided basic insights on the relevance of genes in the HSP70 gene family in tomato plants to adverse conditions of temperature, salinity and drought for further analysis of the potential of HSP70 genes in abiotic stress resistance.

- Scientific papers in referred journals (list): 2 article paper
+ Nam Tuan Vu, Ha Quang Dao, Linh Nhat Nguyen, Ly Han Luu, Hanh Hong Ha, Hue Thu Thi Huynh, Hien Thu Thi Le (2022). Evaluation of the involvement of HSC70 gene in the growth and development of tomato. Vietnam Journal of Science, Technology and Engineering - Series B (ISSN: 1859-4794) (in Vietnamese, accepted).
+ Nam Tuan Vu, Ngoc Bich Thi Nguyen, Hanh Hong Ha, Linh Nhat Nguyen, Ly Han Luu, Ha Quang Dao, Trinh Thi Vu, Hue Thu Thi Huynh, Hien Thu Thi Le (2022). Science Progress 106 (1): 00368504221148843..
- Final report
- Summary of a completed postdoc’s project (in English)
- Summary of a completed postdoc’s project (in Vietnamese)