- Update the status distribution of species C. balansae at four different ecological regions of Vietnam
- To develop a set of EST-SSR markers for the studies of population genetics of C. balansae species;
- To use these markers to assess the genetic diversity and population genetic structure of the endangered C. balansae species.

Theoretical results:
+ Results study showed current status of the population and species of C. balansae  in 9 different geographical regions of Northern Vietnam.
+ A total of 15 EST-SSR markers were developed from the transcriptome dataset
+ We used a set of 15 microsatellites to analyze 161 adult trees from nine different populations, representing the geographical distribution of C. balansae. Our results showed a low genetic diversity level with an average Ho = 0.246 and He = 0.262, and a high level of genetic differentiation (FST = 0.601). The bottleneck tests indicated evidence of a reduction in the population size of the two populations (TC and CP). Additionally, all three clustering methods (Bayesian analysis, principal coordinate analysis and Neighbor-joining tree) were identified in the two genetic groups. The Mantel test showed a significant positive correlation between genetic distance and geographic distance (R2 = 0.7331).
+ The Maxent model for C. balansae distribution showed a good performance (test AUC = 0.861), compared with null model (test AUC = 0.535). The most suitable area for current distribution of C. balansae was 2776.9 km2, occupying about 1.7% of the study site. Our data suggest that the most suitable area of C. balansae could increase in both RCP (Representative Concentration Pathway) 4.5 and RCP 8.5 scenarios. In the future, three northwest provinces of Vietnam could be new suitability areas for C. balansae distribution
Applied results:
This study will provide a platform for the conservation of C. balansae both in ex-situ and in in-situ plans.

+ Update the status distribution of species C. balansae at nine different ecological regions of Vietnam
+ The first time study, A total of 15 EST-SSR markers were developed for species C. balansae. These microsatellite markers will provide platform for study of genetic diversity and population structure of this species at nine different ecological regions of Vietnam.
+ Our results showed a low genetic diversity level and a high level of genetic differentiation. The bottleneck tests indicated evidence of a reduction in the population size of the two populations. Additionally, all three clustering methods (Bayesian analysis, principal coordinate analysis and Neighbor-joining tree) were identified in the two genetic groups. The Mantel test showed a significant positive correlation between genetic distance and geographic distance.
+ Our data suggest that the most suitable area of C. balansae could increase in both RCP (Representative Concentration Pathway) 4.5 and RCP 8.5 scenarios.  In the future, three northwest provinces of Vietnam would be new suitability areas for C. balansae distribution.
+ The project has published 3 paper;
+ The project has supported 02 students master.

- Scientific papers in referred journals (list): 03 Papers
- Other products (if applicable): has supported 02 students master