Identification and evaluation of distribution, biological activities (antibacteria and anticancer) of endophytic actinomycetes from mangrove plants (Bruguiera gymnorrhiza, Avicennia marina, Agiceras corniculatum) collected in Quang Ninh province.

Theoretical results:
A total of 62 endophytic actinomycetes was isolated from Avicennia marina, Agiceras corniculatum, and Bruguiera gymnorrhiza collected in Quang Ninh province.
Out of 62 endophytes, strain SX6 from A. corniculatum and MX9 from B. gymnorrhiza showed significant antibacterial activity against at least 4 tested bacteria with inhibition zones ranging from 7,96-32,5 mm. Besdies, ethyl acatate extract of both strains also displayed cytotoxic activity against 3 cancer cell lines including A549, MCF7, and Hep-3B.
Strains SX6 and MX9 were identified as S. parvulus SX6 and S. parvulus MX9, respectively. S. parvulus MX9 was deposited at VAST-Culture Collection of Microorganisms under accession number VCCM 22513.
S. parvulus SX6 and S. parvulus VCCM 22513 were sequenced by Illumina platform in order to study genetic basis involved in antibacterial and anticancer properties. The genome of S. parvulus SX6 contained 04 novel gene clusters atttributed to antibacterial and anticancer activities. In addition, biosynthetic pathways of plant-derived compounds such as daizdein and genistein were proposed for the first time. On the other hand, adaptation of S. parvulus VCCM 22513 to endophytic lifestyle was demonstrated using genome mining.
Applied results: S. parvulus SX6 could be an effective platform for daizdein and genistein production which could be applied in medicinal and pharmacetutical industries. These findings promote employing whole-genome sequencing in finding new metabolites with biological activities. 

The project exploited for the first time biosynthetic pathways of daizdein and genistein from endophytic S. parvulus SX6.The underlying mechanisms involved endophytic lifestyle of S. parvulus VCCM 22513 from from B. gymnorrhiza were proved for the first time. These could serve as reference genomes for domestic and international studies. 

Products of science and technology
-    Collection of 43 endophytic actinomycetes
-    02 endophytic strains with strong antibacterial and anticancer activities
-    02 genome sequences
Scientific papers
02 articles in ISI journals:
Ngoc Tung Quach, Thi Hanh Nguyen Vu, Thi Lien Bui, Thi Thanh Xuan Le, Thi Thu An Nguyen, Cao Cuong Ngo, Quyet-Tien Phi. Genomic and physiological traits provide insights into ecological niche adaptations of mangrove endophytic Streptomyces parvulus VCCM 22513. Annals of Microbiology 72, 27 (2022) (IF 3,168).
Ngoc Tung Quach, Thi Hanh Nguyen Vu, Thi Lien Bui, Anh Tuan Pham, Thi Thu An Nguyen, Thi Thanh Xuan Le, Thi Thu Thuy Ta, Pravin Dudhagara, Quyet-Tien Phi (2022) Genome-guided investigation provide new insights into secondary metabolites of Streptomyces parvulus SX6 from Aegiceras corniculatum (L.) Blanco. Polish Journal of Microbiology (Accepted; IF 2,019).
02 articles in pretigous national journal/conference:
Quách Ngọc Tùng, Bùi Thị Liên, Vũ Thị Hạnh Nguyên, Nguyễn Văn Thế, Phạm Anh Tuấn, Phí Quyết Tiến (2021) Đánh giá hoạt tính kháng khuẩn và phân loại chủng xạ khuẩn Streptomyces geysiriensis SX35 phân lập từ cây sú (Aegiceras corniculatum). Hội nghị Công nghệ sinh học toàn quốc 2021: 513-518. ISBN 978-604-9987-88-5
Quach Ngoc Tung, Bui Thi Lien, Vu Thi Hanh Nguyen, Nguyen Thi Thu An, Hoang Ha Chu, Phi Quyet Tien. Endophytic actinomycetes from mangrove plant Avicennia marina in Quang Ninh province, Vietnam: Distribution, cytotoxicity, and antioxidant activities. Academia Journal of Biology (Chấp nhận đăng)
Training results
Master student, Pham Anh Tuan, majoring in Experimental biology (8 42 01 14). Disertation entitled “Biological activities of endophytic actinomycetes from Aegiceras corniculatum collected in Quang Ninh province”
Bachelor student, Vu Duc Truong, course 2021-2022, majoring in Biotechnology at Vietnam Academy of Agriculture. Disertation entitled “Identification, antifungal activity, and plant-growth promotion of endophytic actinomycetes from Aegiceras corniculatum”