Vietnam Academy of Science and Technology

Project's information

Project's title	Using microfluidic system to investigate the dehydration process of micro-droplet of protein solution.
Project’s code	GUST.STS.ĐT2018-HH05
Research hosting institution	Graduate University of Science and Technology
Project leader’s name	Dr. Pham Van Nhat
Project duration	01/06/2018 - 30/06/2021
Project’s budget	3000 million VND
Classify	Fair
Goal and objectives of the project	There are two main goals for this project. Firstly, a completed fabrication system for microfluidic channels in typical laboratory in Vietnam was obtained with reasonable cost, simple and high quality microfluidics. The second one is using microfluidic system to investigate several major conditions for dehydration process of protein solution which includes: time, initial diameter of droplet and initial concentration of protein, the saturation ratio of water in surrounding medium. Consequently, the hydration and osmotic pressure as a function of volume fraction were determined and presented. Moreover, simulation methods were studied and applied to predict the outcomes and to improve the control of dehydration.
Main results	Theoretical results: The project proposed a novel method which using microfluidic system to investigate the dehydration process of protein solution. This method is based on the solvent exchange principle across the interface of droplets. The equation of state of Lysozyme solution was obtained successfully which presented a good agreement to theoretical Carnahan-Starling calculation and experimental data from other previous publications. Applied results: Microfluidic fabrication and applications of its will be able to apply to many research field in Vietnam.
Novelty and actuality and scientific meaningfulness of the results	The novelty of this project is applying the microfluidic system will bring several advantages as high throughput in short time, loss of materials is reduced as well as the cost for each experiments.
Products of the project	- Scientific papers in referred journals (list): 1. PHAM, Van Nhat, RADAJEWSKI, Dimitri, RODRÍGUEZ-RUIZ, Isaac and TEYCHENE, Sebastien. Microfluidics: A Novel Approach for Dehydration Protein Droplets. Biosensors. November 2021. Vol. 11, no. 11, p. 460. DOI 10.3390/bios11110460. - Technological products (describe in details: technical characteristics, place): + A simple, efficiency microfluidic system which surface modification to generate oil in water droplets. + The equation of state for Lysozyme protein solution + The Matlab program to simulate the dehydration process.
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