Project's information

Project's title Research on chemical constituents and cytotoxic activity from Phlogacanthus turgidus (Fua ex Hook.f.) Lindau, product orientation for anti-cancer
Project’s code VAST04.06/19-20
Research hosting institution Institute of Chemical Technology
Project leader’s name Assoc. Prof. PhD. Mai Dinh Tri
Project duration 01/01/2019 - 31/12/2021
Project’s budget 600 triệu đồng
Classify Excellent
Goal and objectives of the project

- Isolation, structural determination, and cytotoxic activity of compounds from Phlogacanthus turgidus.
- Create products for the study of cytotoxic activity against some cancer cell lines.

Main results

Theoretical results:
+ Isolated and determined the structure of 20 compounds: turgidol (1), lupeol (2), lupenone (3), betulin (4), betulinic acid (5), taxarexol (6), tangeretin (7), diosmetin (8), luteolin (9), aloe-emodin (10), emodin 11-O-α-L-rhamnopyranoside (11) (+)-dehydrovomifoliol (12), (3S,5R,6R,7E,9S)-megastiman-7-ene-3,5,6,9-tetrol (13), β-sitosterol (14), daucosterol (15), (+)-syringaresinol (16), harpagoside (17), 10-hydroxyaloin A (18), 10-hydroxyaloin B (19), and martynoside (20). In particular, all these substances were new in the Phlogacanthus turgidus species. Structure (1) has still been identified as a new compound. For the first time, compounds 6, 7, 8, 10, 11, 12, 13, 18, and 19 from the Phlogacanthus genus have been reported, and the presence of anthraquinones and anthrones in the Phlogacanthus genus were, too.
+ The cytotoxic activity on 4 cancer lines: Hep3B, A549, Hela, and MCF-7 was showed that the extract with EtOH, CHCl3, and EtOAc gave IC50 from 26.85 to 89.13 mg/mL.
+ The cytotoxic activity of compounds 2, 3, 4, 5, and 20 on three cancer lines: MCF-7, Hela, and HepG2, were shown and gave IC50 from 3.52 to 25.34 μg/mL. Next, compound (10) against breast cancer line MCF-7 and liver HepG2 with IC50 values of 78.18 and 84.56 μg/ mL, respectively, while compound (1) gave cytotoxic to MCF-7 with IC50 of 81.23 μg/mL
+ Developed a product process on extracts with cytotoxic activity. Conditions of the process: solvent of EtOH 70%, solid/liquid ratio of 1:12, extraction temperature of 80oC, time of 120 minutes..
+ Developed basic standards of products.
+ The safety of the product has been evaluated, and if the dose is 24.0 g of dry extract/kg, it is no happen acute toxicity.

Novelty and actuality and scientific meaningfulness of the results

For the first time, the chemical constituent and cytotoxic activity of Phlogacanthus turgidus (Fua ex Hook.f.) Lindau was investigated. A novel substance and purified others were isolated and tested for cytotoxicity from the extract of this tree.

Products of the project

- Scientific papers in referred journals (list):
1. Mai Dinh Tri, Nguyen Tan Phat, Nguyen Tien Trung, Cam-Tu D. Phan, Phan Nhat Minh, Mai Thanh Chi, Thi-Phuong Nguyen, Chi Hien Dang, Luu Hong Truong, Nguyen Kim Tuyen Pham, Tran Thi Ngoc Mai & Thuc-Huy Duong, (2020). A new 26-norlanostane from Phlogacanthus turgidus growing in Vietnam, Journal of Asian Natural Products Research,
2. Phan Nhat Minh, Nguyen Tan Phat, Mai Thanh Chi, Dang Chi Hien, Mai Dinh Tri, (2021).  Chemical constituents of the whole plant of Phlogacanthus turgidus (Fua ex Hook.f.) Lindau, Science & Technology Development Journal – Natural Sciences, 5(2):1341-1349
- Patents (list):
- Technological products (describe in details: technical characteristics, place):


- Investigate the chemical composition of the remaining fractions.
- Evaluation of anti-inflammatory and glucosidase enzyme inhibitory activities on extracts and purified substances.
- Investigate extraction conditions to remove impurities and improve the main active ingredients.
- Develop a lupeol quantification process and semi-synthesis derivatives from this substance.

Images of project