Project's information

Project's title Synthesis and study of the effect of metal nanoparticles on the growth and disease resistance of wheat and barley in the pre-sowing treatment of seeds
Project’s code QTBY02.02/19-20
Research hosting institution Institute of Materials Science
Coordinating unit, co-chair Institute of Experimental Botany named after V.F. Kuprevich of the National Academy of Sciences of Belarus
Project leader’s name Dr. Vu Hong Son
Project duration 01/04/2019 - 30/04/2022
Project’s budget 200 million VND
Classify Excellent
Goal and objectives of the project

- To synthesize metal nanoparticles Fe, Cu, Co, ZnO with size 40-100 nm and silver nano solution with concentration from 100 - 500 ppm.
- To evaluate the effects of metal nanoparticles on the growth and disease resistance of wheat and barley plants when used for seed treatment.

Main results

Metal nanoparticles were synthesized with corresponding sizes: iron nanoparticles with sizes of 40-100 nm; copper nanoparticles - 10 - 70 nm; Co nanoparticles - 50 -100 nm. The ZnO nanoparticles prepared by sol-gel method has a small, uniform spherical particle size. The ZnO nanoparticles size is concentrated in the range of 10-50 nm. The fabricated ZnO nanoparticles has a rather high surface area, a certain porosity and an average pore diameter. The content of Zn and O in the fabricated samples was determined to reach 86.75% by mass. Besides, solution of silver nanoparticles was successfully synthesized with the average size in the range of 5-20 nm, uniform particles with little cohesion. The average size of particles in the range of 44 nm by DLS analysis method and zeta potential in the range of 33mV. The results showed that the synthesized samples have similar grain sizes as well as zeta potential, so the repeatability of the fabrication process is highly stable.
When applying metal nanoparticles made from Vietnam in the pre-sowing treatment of seeds, Belarusian scientists have observed their positive effects on the growth of cereal crops. These effects depend on the concentration of nanoparticles used. At medium and high concentrations of nano-metals Fe0, Cu0, Co0, the length of the above-ground part of the wheat seedlings was increased by 14 - 22%. Nano-particles of ZnO and solution of chitosan-Ag had weaker, only from 6 to 10%. The impact on barley seedlings was weaker. The height of the above-ground part of barley seedlings increased by 6-9% compared to the control values. It should be noted that the amount of particles was very small, 0.5 mg/kg of grains. Most of the metal content are built up in the surface to the seedling shells and not the crop product.
A visible antifungal effect of metal nano-particles on phytopathogenic fungi was observed. The infection rate of untreated cereal grains was 44% for barley and 28% for wheat. Pre-sowing treatment of wheat grains with metal particles reduced the number of infected grains by 1.75 to 2 times. Stronger suppression of the development of phytopathogenic microflora was observed on barley grains. The number of infected grains decreased by 1.8 to 3.6 times in all experimental conditions. The treatment also increased the synthesis of photosynthetic pigments and inhibited membrane lipid peroxidation. The decreased contents of water-soluble substances from plant tissues indicate an adaptive-protective mechanism
The analysis of the crop structure showed that the pre-treatment of grains with nanoparticles had substantial effects on stems and grains.  The greatest improvement was observed with Fe and Co nano-particles. These nano-metals increased the average mass of stems by 20 –60% and the mass of ears by 20–50%. The results can provide a promising economical and environmentally friendly pre-sowing treatment with ultra-low concentrations of metal nanoparticles.
From the positive results obtained by the Belarusian partner research group, and based on the previous results obtained by the Vietnamese research team when applying metal nanoparticles on plants grown in Vietnam, such as corn, turmeric, ginger, it is possible to apply the results of the project on other crops in Vietnam (for example, on wet rice), as well as in other plants in Belarus.

Novelty and actuality and scientific meaningfulness of the results

The obtained results of the study showed that the treatment of wheat and barley seeds with metal nanoparticles and metal oxides had a positive effect on the growth of wheat and barley seedlings (increasing stem height of wheat, barley plants). The nano-preparations also showed antifungal activity against phytopathogenic fungi, reduced the infection rate of wheat and barley grains, increased the synthesis of photosynthetic pigments and inhibited membrane lipid peroxidation, and increased the average mass of stems by 20–60% and ear mass by 20–50%. The results can provide a promising economical and environmentally friendly presowing treatment with ultra-low concentrations of metal nanoparticles.


Products of the project

- Publications:
1. Son A. Hoang, Hanh H. Cong, Shukanov V. P., Karytsko L. A., Poljanskaja S. N., Melnikava E. V., Mashkin I. A., Huyen T. Nguyen, Khanh D. Pham and Chi M. Phan. Evaluation of metal nano-particles as growth promoters and fungi inhibitors for cereal crops/ Chem. Biol. Technol. Agric. (2022) 9:12
2. Shukanov V.P., Korychko L.A., Melnikova E.V., Polyanskaya S.N., Mashkin I.A., Cong H.H., Vu H.S., Hoang A.S. Changes in some physiological and biochemical indicators for stress tolerance of spring barley under the influence of pre-sowing treatment of seeds with metal nanoparticles/ Biologically active preparations for plant growing: scientific background – recommendations – practical results. Proceedings XVI International scientific-applied conference. – Minsk. – October 22, 2020.
3. Hong Hanh Cong, Hong Nhung Nguyen, Thi Huong Tran, Que Chi Tran, Duy Khanh Pham, Kartsko Larisa Alexandrovna, Shukanov Vladimir Petrovich, Anh Son Hoang. Antifungal activity of silver nanoparticles agaisnt phytopathogenic fungi in vitro/ Vietnam Science and Technology Magazine, 2020 - 9(62) – PP 42-47.
- Other products: 01 bachelor graduated


With the positive results obtained from the research of the Belarusian partner group during the cooperation process, it is possible to see the prospects for the development of nanotechnology applications in seed treatment before sowing on many different crops, in both Vietnam and Belarus. Both research groups need to continue to build larger-scale studies, as well as carry out more in-depth goals and contents to elucidate the mechanism of metal nanoparticles to plants, plant cells and phytopathogenic fungal cells. In addition, the Vietnamese side needs to rely on that to conduct research and application on domestic plants, for example, wet rice in our country.

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