- To clarify natural antioxidant compounds which are able to scavenge ROS from Physalis genus in Vietnam
- To receive and set up ROS scavenging method using using photo-inducible ROS-generating proteins and cytotoxic nanomaterials from Korea partner

- Setting up the 1O2 scavenging method using photo-inducible ROS-generating miniSOG protein model and initially receiving miniSOG gene transferred cells;
- By using the miniSOG+TMB photo-inducible ROS model, we evaluated 1O2 scavenging antioxidant activties of 8 extracts and 8 pure compounds isolated from Physalis angulata and Physalis minima in Việt Nam;
- The compound rutin (PM5) obtained from P. minima presented a strong anti-ROS activity by scavenging 1O2 with the IC50 value at 9.7 µM;
- Beside, the total extract of P. Angulata (PA) exhibited the best cellular protective activities against terbi nanorod toxic induced HEK-293A cells with the cell survival as 12.91 ± 1.22%;
- The water extract of P. angulata showed no hepatoprotective effects in CCL4 oxidative stress induced mice at 100 and 300 mg/kg body weight dosages.

- Setting up the 1O2 scavenging method using miniSOG protein to evaluate antioxidant activities;
- By using the miniSOG+TMB photo-inducible ROS model, we evaluated 1O2 scavenging antioxidant activties of 8 extracts and 8 pure compounds isolated from Physalis angulata and Physalis minima in Việt Nam;
- As the first time, the compound rutin (PM5) obtained from Physalis minima of Vietnam was determined presented a strong anti-ROS activity by scavenging 1O2 with the IC50 value at 9.7 µM.

- Publications:
3.    Kim, M., Kim, E.H., Pham, T.H.Y., Hoang L.T.A, Do T.P., Nguyen T.N., Nguyen T.C., Trieu H.P., Do T.T., Kim Y.P. Colorimetric Determination of Singlet Oxygen Scavengers Using a Protein Photosensitizer. BioChip J. 2020, 14, 148–157; doi:10.1007/s13206-019-4203-6 (SCIE) (ISSN:2092-0280)
4.    Pham T H Yen, Nguyen T Nga, Trieu H Phuong, Nguyen T Cuc, Do T Phuong, Hoang L T Anh, Do T Thao; Determination of apoptotic inductive activities of Physagulin P from Physalis minima plant in Vietnam; Vietnam Journal of Biotechnology, 2021; (in print process)
- Patents:
- Products (description...):
- Other products: supporting 1 PhD student

Conclusion:
- We have completed all proposed objectives, contents and set up miniSOG+TMB photo-inducible ROS model to evaluate the 1O2 scavenging;
Recommendations:
- We propose to be extending supported from International Cooperative Devsion, VAST for continuing the scientific collaboration with Prof. Young-Pil Kim, NanoBiology Lab, Dept. of Life Science, Hanyang University in order to evaluate potential anti-ROS activities from Vietnamese plants using miniSOG gene transferred cells.