Project's information

Project's title Research of CAR-T containing anti-CD47 scFv h (CD28-41BB/OX40-CD3ζ) receptor targeting leukemia blood cancer cells
Project’s code VAST02.03/18-19
Research hosting institution Institute of Biotechnology
Project leader’s name PhD. La Thi Huyen
Project duration 01/01/2018 - 31/12/2020
Project’s budget 600 million VND
Classify Excellent
Goal and objectives of the project

Building growth protocol for Anti-CD47-Specific CAR-T (scFv(CD47)-CD28-41BB-CD3ζ CAR-T) cells which can be applied in cancer treatment.
Main results

Theoretical results: Project made creating protocol of humanize scFv based on antibody sequences which were in Kabat theory publication, designed successfully structure of Chimeric Antigen Receptors with bioactivity, separated and isolated lympho T cells, created lympho CAR-T cells containing CAR by gene delivery and CAR-T cells with effectively killing cancer cells. There were 2 publications: 1 paper in Vietnam Medical Journal and 1 in Journal of Immunology Research (SCIE), IF > 3. Besides, 1 patent was registered and accepted; in education, 1 graduated master, 1 bachelor of biology in VNU University of Science was guided.
Applied results: Based on protocol created in project, it can be applied for different targets: creating specific scFc with different antigens; creating CAR-T cells or Chimeric Antigen Receptors cells target different markers; if further study is done, CD47 CAR-T cells can be applied in cancer which is in liquid or solid form.
Novelty and actuality and scientific meaningfulness of the results

In Vietnam, this is the first time which Anti-CD47-Specific CAR-T Cells were created with high efficiency for broad spectrum cancer killing.
Products of the project

- Scientific papers in referred journals (list):
1. Huyen Thi La, Dao Bich Thi Tran, Hai Manh Tran, Linh Trong Nguyen, "Third-Generation Anti-CD47-Specific CAR-T Cells Effectively Kill Cancer Cells and Reduce the Genes Expression in Lung Cancer Cell Metastasis", Journal of Immunology Research, vol. 2021, Article ID 5575260, 13 pages, 2021. https://doi.org/10.1155/2021/5575260.
2.    La Thi Huyen*, Tran Thi Bich Dao, Tran Manh Hai, Nguyen Trong Linh (2020). Building the phage display SIRPα library from mutation and recognizing the mutant containing high affinity with CD47. Vietnam Medical Journal, vol.496, p. 199-205.
- Patents (list):
1.    La Thi Huyen, Tran Manh Hai, Tran Thi Bich Dao. Patent, Decision no.2403w/QĐ-SHTT on 09/02/2021. Title: CD47-resistant scFv and CAR-T receptor mosaic T-lymphocyte used in cancer treatment.
- Technological products (describe in details: technical characteristics, place):

Protocol of creating CAR-T cell which contains Specific CD47 scFv receptor: scFv(CD47)-CD28-41BB-CD3ζ Protocol accepted
Gene encoding CD47 scFv Storage in plasmid pcDNA3.1, -20oC
Lentivirus packaging gene which encodes CD47 scFv: scFv(CD47)-CD28-41BB-CD3ζ Storage in liquid phase nitrogen
Anti-CD47-Specific CAR-T (scFv(CD47)-CD28-41BB-CD3ζ CAR-T) with anti-cancer Immune Response Storage in liquid phase nitrogen
Images of project
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