Project's information

Project's title Cell cultures creation and investigation of pharmacologically valuable phenolic compounds and terpenoid indole alkaloids biosynthesis in a various varieties of Catharanthus roseus Vietnamese flora in vivo and in vitro. Phase 1st: Research on in vitro cell culture system from C. roseus samples of Vietnam for developing cell lines producing high alkaloids content.
Project’s code QTBY01.05/18-19
Research hosting institution Institute of Marine Biochemistry (IMBC)
Coordinating unit, co-chair Belarusian Republican Foundation for Fundamental Research.
Project leader’s name Dr. Tran My Linh
Project duration 01/01/2018 - 31/03/2021
Project’s budget 200 million VND
Classify Excellent
Goal and objectives of the project

The aim’s  project is to produce Catharanthus roseus callus and suspension cell lines from C. roseus varieties of Vietnam, which contain high content of total alkaloids and important secondary metabolites

Main results

Research and application: a quality callus for suspension culture of cells was successfully established from stem fragments of two C. roseus varieties (QN02 and H01) and multiplied on MS medium supplemented with 1.5 mg/L kinetin and 1.5 mg/L 2,4-D. Cell suspension cultures were maintained by subculturing using 150 mg initial cell inoculum/50 mL, a medium pH of 5.5 and culture temperature of 25°C. The low alkaloid accumulation in this system was compensated for by using cell extracts of the endophytic fungi Fusarium solani RN1 and Chaetomium funicola RN3. The most important finding is that the fungal treatments elicited the accumulation of one tested bis-indole alkaloid, vinblastine. The obtained results are the basic for further investigation on improving biosynthesis of valuable compounds in C. roseus in vitro suspension cells.
Publication and education: The above results were published in 01 SCIE paper (Plants) and a manuscript is submitted to the International Journal of Advanced Research (IJAR). The project also supported in training 01 master student
Cooperation: In frame of the project, Vietnam researchers had opportunities for discussion about related research fields, enhancing collaboration between Institute of Marine Biochemistry (IMBC) and Belarusian partners (Belarusian State University, Belarusian Central Botanical Garden).

Novelty and actuality and scientific meaningfulness of the results

A quality culture system of C. roseus suspension cell was successfully established from C. roseus varieties in Vietnam. Moreover, four endophytic fungal strains were isolated from C. roseus root samples. The chemical analysis showed that endophytic fungal elicitors stimulated accumulation of alkaloid content and vinblastine in C. roseus cell lines.

Products of the project

- Publications: 01 SCIE paper, 01 paper in international journal.
- Products: tissue cell culture, fungal isolates (IMBC)
- Other products: support in training 01 Master student

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