-    Study on synthesis of some bio-nanocomplexes between antigen proteins and diamond nanoparticles (nanodiamonds).
-    Characterization of the synthesized bio-nanocomplexes.
-    Investigate the ability of nanodiamonds to boost antigenicity of antigen: effects of particle sizes and synthesis procedure.

-    Validation and optimization of conditions to conjugate recombinant H5.c2 and S1/PEDV onto the surface of different diamond nanoparticles.
-    Characterization of synthesized bio-nanocomplexes: biological activity and physiochemical properties (size, zeta potential).
-    Effects of particle size and surface properties of nanodiamonds on the antigenicity of free HA/H5N1 vs HA/H5N1:ND complexes.
-    Training 1 Master student and 1 Bachelor student.
-    Publications: 01 domestic paper, 01 SCI papers.

- This is the first work studying the affinity and immune boosting ability of different types of diamond nanoparticles toward protein antigen. Obtained results showed that commercial “detonation” of ~40 nm and positively-charges nanodiamonds (ND40) do not conjugate H5.c2 antigen. ND40 does not have any immune effects on the antigen in vivo. In contrast, our oxidative NDs (ND40 ~ 40nm, ND100 ~ 100nm) easily form stable bio-nano complexes with the protein antigen in PBS buffer. In vivo experiments found that much more specific and neutralizing antibodies are produced by mice when injection with H5.c2:ND complexes than injection with free H5.c2. And the two NDs has the same effects.
- The use of our oxidative NDs (ND100 ~ 100nm) in protecting antigen protein: the antigenicity of H5.c2:ND100 is maintainded ~ 80% after 7 day- storage at room temperature and ~100% after 7 day- storage at 4oC.

- Scientific papers in referred journals (list): 01 national paper, 01 international paper (SCI-E);
- Patents (list): no
- Technological products (describe in details: technical characteristics, place):
+ Protocol to conjugate H5.c2 and S1/PEDV onto the surface of ND40 và ND100, archived in IBT-VAST.
+ Results: bio-physiochemical characterization of bio-nanocomplexes including H5.c4:ND40, H5.c2:ND100, S1/PEDV:ND100.
+ Results of in vivo experiments in mice: specific and neutralizing antibody measurement of mices injection with free H5.c2 vs H5.c4:ND40, H5.c2:ND100.