Project's information

Project's title Research, application and technology transfer for exploitation, conservation, and development of products from selected species of Ganoderma lucidum (Gannoderma spp.) in the Tay Nguyen region
Project’s code TN18/C10
Research hosting institution other Ho Chi Minh City Institute of Drug Quality control
Project leader’s name Assoc.Prof.Dr. Tran Viet Hung
Project duration 01/07/2018 - 31/03/2021
Project’s budget 20,350.000 million VND
Classify Excellent
Goal and objectives of the project

-    Investigate, evaluate, and study the possibility of developing genetic resources of some valuable species of Ganoderma lucidum (Ganoderma spp.), another name Lingzhi musroom (chineses) or Reishi musroom (Japanese) in the Tay Nguyen region.
-    Research on propagation, development and application to create 4 products from Ganoderma species (Ganoderma spp.) in the Tay Nguyen region
-    Technology transfer, serving the exploitation, conservation and development of some species of Ganoderma lucidum (Ganoderma spp.) in the Tay Nguyen region

Main results

Scientific results:
-    Samples were collected and investigated at 8 provinces: Gia Lai, Dak Lak, Dak Nong, Lam Dong, Kon Tum, Quang Nam, Khanh Hoa, Dong Nai. In the project, we have collect 100% sample according to the project plan as well as surveying 400 questionaires at the mentioned provinces and their surrouding. The investigating researchers group has collected informations following the 4 main categories: characteristics, exploitation, price and origin, quality and usage of Ganoderma.
-    43 species of Ganodermataceae mushroom were identified, including 11 species of the Amauroderma genus and 32 species of the Ganoderma genus among more than 1000 samples collected. The project built their dataset of ecological information, morphological, and color images. From 43 identified species, the research team surveyed over 10 species that have been published with good biological effects to conduct testing such as chemical composition surveys, thin layer chromatography fingerprints, and liquid chromatography. The team also conduct evaluation of biological effects on hepatoprotective, hypoglycemic, lipid-lowering and immune-regulatory effects. Through biological effects, it is possible to preserve and propagate some species such as Ganoderma applanatum, Ganoderma lucidum. These are precious genes that will bring contribution to medical and economic value in the future.
-    The project has implemented a conservation model, 60,000 m2 in area, under the forest canopy with humidity control to stimulate the growth of Ganoderma lucidum in Buon Ea-Ma area, Krong-Na commune, Buon Don district, Dak Lak province.
-    The project has built cultivating procedures of rich content of polysaccharides and rich content triterpenoids of Ganoderma lucidum. The study highlights the possibility of controlling the content of the two main groups of active ingredients in Ganoderma lucidum species, thereby allowing proactiveness in selecting cultivars for medical treatment. Also registered and obtained Viet-Gap certificate for the farming area in Krong Na, Buon Don district, Dak Lak province.
Applicational significance:
-    Project TN18/C10 products from the indigenous Lingzhi source increase the economic value of Ganoderma lucidum and stabilize the output for this medicinal herb, including: 3 types of tonic wine products: Bao Linh, Tu Linh and, Linh Chi liquors; 01 Lingzhi tea product, 01 softgel capsules (Linstata soft capsules), and 03 Ganoderma lucidum products including: total extract, triterpenoid rich extract and polysaccharide rich extract.
-    Lingzhi mushroom farming model can control the content of two main groups of active ingredients, helping to improve the value of Ganoderma lucidum compared to previous agricultural farming models. The project signed a technology transfer and farming training agreement with 2 units in the Tay Nguyen region, 1 facility in the Southwest region; the project also transferred products to 3 facilities, including 1 media company, 2 pharmaceutical manufacturers.
-    Conducted trademark protection for thematic products; The showroom décor and dispaly of Dat Viet Linh Chi aims to bring the products of Ganoderma lucidum in the Tay Nguyen into a typical product for Dak Lak in particular and the Central Highlands Tay Nguyen in general. Mothan 10 tradmark of Lingzhi (Linh Chi Dat Viet, Linh Chi House and mothan 10 tradenames products are protected by licese low in Vietnam.

Novelty and actuality and scientific meaningfulness of the results

-    A conservation model under the forest canopy with humidity control to stimulate the growth of Ganoderma lucidum.
-    Viet Gap farming model and distinctive products of Garnoderma ludicum.

Products of the project

-    Scientific papers in referred journals (list):

Pharmacology journal 9/2017 (number 497 year 57)
Number Name of Paper Journal name
1 Domestic journals
01 Isolation of some triterpenoids from Ganoderma lucidum (Ganoderma lucidum) to establish reference standards Medical journal number 15 - 02/2021
02 A study of Supercritical Fluid Extraction for Garnoderma lucidum Medical journal number 15 - 02/2021
03 Investigation of ganoderic acid A content in some samples Ganoderma lucidun (Leyss ex Fr.) Karst) by high performance liquid chromatography (HPLC)
04 Investigation of polysaccharide content in some samples of Vietnamese Ganoderma lucidum (Ganoderma lucidum (Leyss. ex Fr.) Karst.) by photometric method. Pharmacology journal 9/2018 (number 509 year 58)
2 International journals  
01 Lanostane Triterpenoids from Ganoderma tropicum Collected in Vietnam and Their Nitroblue Tetrazolium Reductive Activity In Vitro

Natural Product Sciences Vol. 26, No. 4, 2020

02 Lanostane-type Triterpenoids from Ganoderma lucidum and G. multipileum Fruiting Bodies

Natural Product Communications
Volume 13, Number 11, 1441 – 1444, 2018

- Patents (list):
Procedure of extraction rich in triterpenoids by supercritical fluid extraction for Garnoderma lucidum
Procedure of extraction and isolation Lucidenic acid E2, Lucidenic acid N and Ganodermanotriol from Ganoderma lucidum
- Specific products (describe in details: technical characteristics, place): 5 reference standards isolated from Ganoderma lucidum, over 1000 kg of Ganoderma lucidum, Lingzhi extract (3 types), Lingzhi soft capsules, Lingzhi wine (Bao Linh, Tu Linh, Lingzhi). All products are stored at the Deparment of Research and Development, Institute of Drug Quality control Ho Chi Minh City except for 2 models belonging to Tay Nguyen Pharmaceutical Development Joint Stock Company. The 2 models include: Conservation model of some reishi mushroom species (Ganoderma spp.) of the Central Highlands in Yok-Don National Park, Buon Don district, Dak Lak province and Reishi mushroom farming model of the Central Highlands in Krong Na commune, Buon Don district, Dak Lak province.
- Other products (if applicable): Procedure of isolation, purification and evaluation of reference standards; Reishi mushroom cultivation process.

Research area

The whole territory Vietnam

Recommendations

-    Recommand to conduct further isolation studies on Ganoderma applanatum species
-    Recomanmd to diversify products from Lingzhi mushoom in order to increase the value of Vietnamese Lingzhi
-    It is necessary to conduct a more extensive assessment of the cultivation of Lingzhi mushoom in Vietnam in order to build the Vietnamese Lingzhi mushoom into a national and international brand.