Overall objective:
Evaluating the effects of simulated microgravity on the cytoskeletal alterations using 3D clinostat simulator
Specific objectives:
- Design and manufacturing 3D clinostat microgravity simulator
- Estimating the effecs of simulated microgravity on the proliferation and cytoskeleton alteration in human messenchymal stem cells, liver cells and fibroblast by using 3D clinostat simulator.

- Product type I (equipment): 3D clinostat was designed and manufactured to induce the simulated microgravity. This 3D clinostat can induce the simulated microgravity from  10-3 G to 0.9 G and operate in CO2 incubator for 72 h. This simulator can be used not only for cell culture research, but also for other biological study on microorganisms, plants and animals.
- Product type II (operating principle reports, methods, design drawings and technology): full quantity and types according registration.
- Product type III (fully published and exceeded): published 4 international articles (2 articles indexed in SCIE), and 2 domestic articles.
Training products (fully accorded and exceeded): completed the training of 1 master and supported the training of 2 PhD students.

Rotating wall vessel (RWV) model. a: structure of the rotary chamber, b: the 3D rotating structure of the rotating chamber, c: the commercial rotary chamber (Synthecon, Incorporated, USA)

Random Positioning Machine (RPM) Model (Netherlands Space Center)

- Design and manufacturing 3D clinostat microgravity simulator which was used for experiments on animal cells.
- Determination of the effects of simulated microgravity on cell proliferation
- Determination of the effects of simulated microgravity on cytoskeletal alterations, including microfilament and microtuble.
- Contributing to training poastgraducate education and publication.

The articles has been published:
1.    Ho Nguyen Quynh Chi, Hoang Nghia Son, Doan Chinh Chung, Le Dinh Huan, Tran Hong Diem, Le Thanh Long (2020) Simulated Microgravity Reduces Proliferation and Reorganizes the Cytoskeleton of Human Umbilical Cord Mesenchymal Stem Cells, Physiological Research, 69: 897-906. (SCI, Q2, IF 1.88).
2.Cell Cycle Regulators and Cytoskeletal Proteins. International Journal of Molecular Sciences. 2021; 22(9):4550. (SCIE, Q1, IF 5.92).
3.    Hoang Nghia Son, Ho Nguyen Quynh Chi, Le Ngoc Phuong Thanh, Truong Thi Han, Nguyen Thai Minh Han, Doan Chinh Chung, Le Thanh Long (2020) Effects of simulated microgravity on the morphology of mouse embryonic fibroblasts (MEFs), Rom Biotechnol Lett. 2020; 25(6): 2156-2160. (Q3, IF 0.823).
4.   Ho Nguyen Quynh Chi and Hoang Nghia Son (2020) Morphological changes of human mesenchymal stem cells under 3d clinostat culture, Asian Jr. of Microbiol. Biotech. Env. Sc.,  22(3): 63-67. (Q4).
5.   Ho Nguyen Quynh Chi, Hoang Nghia Quang Huy, Doan Chinh Chung, Hoang Nghia Son, Le Thanh Long (2020) Inhibited growth of mesenchymal stem cells under simulated microgravity, Journal of Biotechnology 18(2): 257-264.
6.    Hoang Nghia Son, Hoang Nguyen Quang Huy, Tran Thi Bich Tram, Ly Ngoc Cang, Ho Nguyen Quynh Chi, Truong Xuan Dai, Le Ngoc Phuong Thanh, Truong Thi Han, Nguyen Thai Minh Han, Le Thanh Long (2020) Evaluating the effects of simulated microgravity on mouse fibroblast, Academia Journal of Biology 2020, 42(3): 129–134.    
Specific products (describe product, place of storage):