Build procedure of quinonoid extraction from Khanh Hoa sea urchin to orient the production of cosmetics.
Propose the process of cosmetic cream preparation containing quinonoid from Khanh Hoa sea urchin at laboratory scale.

Theoretical results:
- The procedure of quinonoid extraction from Khanh Hoa sea urchin with the scale of 1kg material/a batch has been built with a using solvent H2SO4 10 %, the ratio between material and EtOH solvent ratio, 1:20 (w/v) and number of extracts, 2 times.
- The content and composition of quinonoid pigments from four Khanh Hoa sea urchins including Diadema savignyi (Audouin), Diadema setosum (Leske). Echinochrome A was the major component of Diadema setosum (Leske), Stomopneustes variolaris (Lamarck) and Diadema savignyi (Audouin) (87%, 73% and 59%, respectively) while spinochrome E was the major component of Tripneustes gratilla (Linnaeus) (72%). Besides, other components were spinochrome D, spinochrome A.
- The cosmetic formular and the procedure of cosmetic cream preparation containing quinonoid from Khanh Hoa sea urchin at laboratory scale (0.2 kg/a batch) have been built.
- Extracted quinonoid pigments from four sea urchins and cosmetic cream preparation material containing this quinonoid exhibited biological activities. Highest DPPH scavenging capacity belonged to the quinonoid pigments from Diadema setosum (Leske) with an EC50= 26.82 g/ml. the quinonoid pigment from Tripneustes gratilla displayed the lowest antioxidant activity among the four sea urchins with an EC50 value of 58.47 g/ml. Highest DPPH scavenging capacity belonged to the quinonoid pigments from Diadema setosum (Leske) with an EC50= 26.82 g/ml. the quinonoid pigment from Tripneustes gratilla displayed the lowest antioxidant activity among the four sea urchins with an EC50 value of 58.47 g/ml. The quinonoid pigment from Diadema setosum (Leske) showed the highest inhibition 87.9  4.1% of tyrosinase enzyme while PHNQ pigment from T. gratilla showed the lowest inhibition 68.5  2.9% of tyrosinase at 250 µg/ml. The antioxidant activity of the cosmetic cream containing the Diadema setosum quinonoid pigment was exhibited significantly.
- The cosmetic cream containing quinonoid pigment has not caused skin irritation on the tested mice.
Applied results: 10 bottles of cosmetic cream containing quinonoid extracted from Khanh Hoa sea urchin.

The antioxidant activity of quinonoid pigment extracted from Khanh Hoa sea urchin was exploited to orient towards application in cosmeceuticals.
Cosmetic cream preparation containing natural quinonoid antioxidants have potential in the cosmetic pharmaceutical industry.

Scientific papers in referred journals:

• Vo Mai Nhu Hieu, Tran Thi Thanh Van, Cao Thi Thuy Hang, Mishchenko Natalia P., Fedoreyev Sergey A., Hai Bang Truong, Polyhydroxynaphthoquinone pigment from vietnam sea urchins as a potential bioactive ingredient in cosmeceuticals, Natural Product Communications, Volume 15(10): 1–8,  DOI: 10.1177/1934578X20972525.
• Vo Mai Nhu Hieu, Tran Thi Thanh Van, Bui Minh Ly, Vo Tan Thong, Mischenko N.P, Quinonoid from Khanhhoa sea urchins như as a potential bioactive ingredient in cosmeceuticals, Vietnam Journal of Chemistry, Volumn 57, 4E3,4, 40-44, 2019

Technological products:
- 500 mg quinonoid with crimson powered form is stored at Nha Trang institute of Technology Research and Application.
- The project’s leader confirms hereby the veracity of all the details and information given above.