- The crude extract and fractions derived from methanol extract, such as n-hexane fraction, chloroform fraction, ethyl acetate fraction and water-soluble fraction of Moringa oleifera leaves were successfully collected. Total phenolic and flavonoid contents of extracts or fractions were determined. The total flavonoid content is positive correlation with total phenolic content in extracts or fractions. As a result, ethyl acetate fractioncontained noticeable amount of phenolic acids and flavonoids.
- Methanol extract andethyl acetate fraction inhibited the proliferation of both human melanoma A375cells and A2058 cells, but had little effect on WS1 normal human skin fibroblasts and primary normal human dermal fibroblasts (NHDF).
- The phenolic-rich ethyl acetate fraction markedly inhibited the growth of A375 cells in a dose- and time-dependent manner. The anti-proliferation was supposed to be mediated via apoptosis, which was demonstrated by the significant increase of condensed chromatin, DNA fragmentation and PS externalization.The phenolic-rich ethyl acetate fraction exerted significant anti-cancer effects on melanoma cells in vitro which involved in both Caspase-dependent and Caspase-independent apoptosis pathways.
- These results provided a fundament for the using of extracts of Moringa oleifera leaves to treat skin cancer effectively.

The extract from the leaves of Moringa Oleifera Lam. leaves possessed high anti-cancer property against melanoma cells. The mechanism of cell death involved in both Caspase-dependent pathway through activation of Caspase-9 and Caspase-3/7 and through Caspase-independent pathway via AIF translocation.

Technological products: methanol extract, aqueous extract, n-hexane fraction, chloroform fraction, ethyl acetate fraction derived from Moringa oleifera leaves was prepared and characterized. The data for the cytotoxic effect of Moringa oleifera leaf extract against melanoma cells. They were kept at Graduate University of Science and Technology, Vietnam Academy of Science and Technology.
Scientific papers in referred journals: 02, journal in ISI/SCI-SCIE and 01, international science conference  
+ Do BH et al. Mitochondria-mediated Caspase-dependent and Caspase-independent apoptosis induced by aqueous extract from Moringa oleifera leaves in human melanoma cells. Mol Biol Rep. 2020 May;47(5):3675-3689.
+ Do BH et al. Phenolic Extraction of Moringa Oleifera Leaves Induces Caspase-Dependent and Caspase-Independent Apoptosis through the Generation of Reactive Oxygen Species and the Activation of Intrinsic Mitochondrial Pathway in Human Melanoma Cells. Nutr Cancer. 2021;73(5):869-888.
+ Do BH, et al. The phenolic extract of Moringa Oleifera leaves induce apoptosis in human melanoma cells. 3rd Biology Conference - Quy Nhơn 2020.
Educational products: 01 Master of Science, decision of Rector of HCMC University of Food Industry (No: 1199/QĐ-DCT)