To produce probiotic for fish pond water treatment and antimicrobial products for controling black-body disease and hemorrhagic disease on snakeskin gourami fish (Trichogaster pectoralis) to reduce the use of chemicals and antibiotics in the farming process, reducing cultural costs and ensure good quality commercial fish.

- Theoretical results:

• Selected of isolated bacteria from Dong Thap province that able to use for producing probiotic for snakeskin gourami fish pond water treatment and antimicrobial products for controling dark – body and hemorrhagic diseases on snakeskin gourami fish.
• Isolated of Streptococcus agalactiae and Klebsiella pneumoniae  and determined they are causative agents of dark – body and hemorrhagic diseases on snakeskin gourami fish.
• Contributed in traing 2 Masters in Biotechnology.

- Applied results: Compled of three production processes of probiotics with selected bacterial sources in Dong Thap province that can be proactively source and control the quality of the products. Using these products in the farming process will reduce the amount of chemicals and antibiotics, and reduce production costs.

• Bacterial strains isolated and selected from samples collected in Dong Thap can be used to produce specific probiotics for fish pond water treatment and control the black-body and hemorrhagic diseases on snakeskin gourami fish;
• Isolated Streptococcus agalactiae and Klebsiella pneumoniae and determined they are one of the causes of black body and hemorrhagic diseases as the basis for further research for the control of these two diseases on snakeskin gourami fish.
• Established three production processes of probiotics within selected local bacteria sources in Dong Thap to help proactively source and control the quality of products, especially the microbial products for prevention / resistance to black-body and hemorrhagic diseases on snakeskin gourami fish has not available on the market.

- Scientific papers in referred journals (list):

1. Phạm Minh Trung, Huynh Hoan My, Nguyen Nguyen Trinh, Le Quynh Loan, Le Thi Anh Hong (2019). Isolation and selection of nitrifying baacteria from Snakeskin gourami (Trichogaster pectoralis) fish – ponds in Dong Thap province. Proceedings National Biotechnology Conference 2019, Ho Chi Minh National University Publishing House, ISBN 978-604-73-7266-9, page 275 – 279.
2. Pham Thi Minh Ngoc, Vo Van Tuan, Nguyen Hoang Dung, Jaruwan Sidhipol, Le Thi Anh Hong (2019). Initial results of determining the causative agent of hemorrhagic disease in Trichogaster pectoralis, Ho Chi Minh National University Publishing House, ISBN 978-604-73-7266-9, page 592-597.
3. Le Thi Anh Hong, Pham Thi Minh Ngoc, Duong Khanh, Vo Van Tuan, Nguyen Hoang Dung.  Isolation and selection of bacteria inhibiting Streptococcus agalactiae causing dark – body disease on Snakeskin gourami fish (Trichogaster pectoralis).Vietnam Journal of Biotechnology. (Accepted).

- Patents (list): None
- Technological products (describe in details: technical characteristics, place):

• The baterial strains used in the production of probiotic for the treatment of fish pond water are included: Bacillus amyloliquefaciens (amylase biosynthesis), Bacillus siamensis (protease biosynthesis), Bacillus pumilus (lipase biosynthesis), Bacillus amyloliquefaciens (cellulase biosynthesis), Bacillus megaterium (ammonium oxidation), Pseudomonas stutzeri (nitrite oxidation);
• Bacterial strains used in produce probiotics to control black-body and hemorrhagic diseases on snakeskin gourami fish are included: Bacillus subtilis (inhibiting Streptococcus agalactiae  causing black - body disease) and Bacillus amyloliquefaciens (inhibiting Klebsiella pneumoniae causing hemorrhage disease);
• Two strains of bacteria that cause black-body disease and hemorrhagic disease in Snakeskin gourami fish are: Streptococcus agalactiae and Klebsiella pneumoniae.

All bacterial strains are being stored at the laboratoly of Department of Bioconversion Technology, Institute of Tropical Biotechnology and will be transferred to Dong Thap Verification and Testing Center.

- Other products (if applicable):

• Production Process of probiotic for fish pond water treatment in the lab scale (5 liters / day);
• Production process of probiotic for controling black-body disease on snakeskin gourami  fish in lab scale (5 liters / day);
• Production process of probiotic for controling hemorrhagic disease on snakeskin gourami  fish in lab scale (5 liters / day);

Dong Thap Verification and Testing Center.

To continue studying on large scales and proposing to follow the project results, developing into a production project to produce  2 probiotics which are used  for pond water treatment and control on the black - body and  hemorrhage diseases on snakeskin gourami (Trichogaster pectoralis) fish.