Project's information

Project's title Research and creating the rapid kit for detecting of African Swine Fever (ASF) viruses in Vietnam using multiplex PCR
Project’s code ĐL.04/19-21
Research hosting institution Institute of Genome Research
Project leader’s name Assoc. Prof. Vo Thi Bich Thuy
Project duration 01/06/2019 - 30/06/2020
Project’s budget 700 million VND
Classify Excellent
Goal and objectives of the project

Development the fast, accurate, and low-cost diagnostic method using molecular biology (Multiplex PCR) to identify ASFV-positive in swine, and the same time identify the genotype of ASFV circulating in Vietnam.
Main results

-    Theoretical results: Developed: 1) A specific geno
-    me sequence data set for classifying 23 ASFV strains currently circulating in the world; 2) A set of specific genome sequence data to determine Vietnam's ASFV genotype; 3) Multiplex PCR-specific kit for ASFV strains in Vietnam.

 
Novelty and actuality and scientific meaningfulness of the results

For the first time in Vietnam, a successful kit has been developed that can quickly identify the presence of ASFV and identify ASFV genotype currently circulating in the outbreak in Vietnam. In a reaction that integrates multiple primers, enzymes, dNTP mixtures, and buffers, to perform a PCR reaction just add the total DNA sample and diH2O in the required ratio to amplify on the PCR machine, and measure results by electrophoresis agarose gel. The set of biological product is fitting the industry standards / animal standards / vaccine products and veterinary medicine with laboratories and field in Vietnam (QCVN 01-187: 2018 / BNNPTNT; QCVN 01-03: 2009 / MARD; TCVN 8684: 2011; Asean standards for Animal vaccines; However, due to the short time of implementation of the project and limited funding, the subject has not conducted independent testing according to the regulations on testing, testing and quality control of veterinary drugs; TCVN XXXX: 2018).
Products of the project

- Scientific papers in referred journals (list):
- Patents (list):
- Technological products (describe in details: technical characteristics, place):
- Other products (if applicable):
+ ASFV genome sequence data set in raw and processed form using bioinformatics tools (The data is saved as FASTA files)
+ ASFV blood sample set and specimens collected at outbreak localities (300 samples from Ba Vi, Soc Son, Bac Ninh, Thai Binh, Hai Duong, Hung Yen, Yen Bai, and Phu Tho)
+ Total DNA sample has been extracted, tested and passed for the next molecular biology experiments (300 samples).
+ Specific primer set to determine the presence and genotype identification of ASFV in Vietnam (09 primers)
+ Multiplex PCR kit specific for ASFV genotype in Vietnam (300 reactions)

 
Recommendations

1) Further research on other genotypes to complete the ASFV detection and identify genotypes kit.
2) Continue research to produce vaccines against ASFV in Vietnam.