Project's information

Project's title Study on the improvement of artificial breeding and grow-out technique of clownfish Amphiprion ocellaris Cuvier, 1830
Project’s code VAST.SXTN.03/17-18
Research hosting institution Institute of Oceanography
Coordinating unit, co-chair VINA MARINEFARM TECHNOLOGY APPLICATION AND PRODUCTION CO., LTD
Project leader’s name Ho Son Lam
Job Title, The rank of a Professor, Academic degree MSc.
Project duration 01/04/2017 - 30/06/2019
Project’s budget 1.800.000.000 VND
Classify Fair
Goal and objectives of the project
- Goal of the project: Development of nemo anemonefish breeding industry for vietnam ornamental fish 
- Objectives of the project:
(1) Improvement of the technique of artificial breeding and grow-out of clownfish with targets:
- Survival rate of 1 month of age about 50% and market size about 85%. 
- Maximum re-maturation and spawning period: 15 days.
- Rate of egg eaten broodstock below 20%.
- Replacing Artermia in the rearing of fry after the 30th days tages
- Increase profit / cost ratio to 30%. 
(2) Experimental production of 15,000 commercial nemofish.
Main results
- Theoretical results:
Determined the optimum concentration of vitamin E for broodstock diet is 375 mg vitamin E/kg feed which positively influenced  the rate of egg loss and hatching rate of egg, the abnormal rate, survival rate of the 3 days old larvae.
The diet containing 100 – 150 mg/kg astaxanthin may be considered as the best level of astaxanthin supplementation for reproductive performance of broodstock, the rate of egg loss, the abnormal rate, survival rate of the 3 days old larvae in clownfish Amphirion ocellaris Cuvier,1830. 
The current project also reveals that the Selco concentration enriched to live feeds was 100 ppm increased the length, the specific growth rate of length and survival rate of 0- 45 days posthatch larvae.
The appropriated time to change from rotifer to artemia nauplii for larve rearing of clownfish was the 4th day post hatch, which possitive effects on the growth in length and weight of clownfish.
The appropriated time to change from artemia to commercial feed was the 16th day post hatch, which possitive effects on the growth in length and weight, the average weekly weight gain of clownfish. 
At 450 mg/kh Canthaxanthin supplemented in the diet improved growth, survival, color and aesthetics of fish A. ocellaris.
Supplemented 0,1% FOS in broodstock diet could also increased the growth of fish (the length, the specific growth rate in length and weight, the average weekly weight gain) and gut morphological characteristics of fish A. ocellaris.
 
- Applied results:
The project produces 15.000 fishes by Vina Marinefarm Technology Application And Production Co., Ltd. The fishes achieved the size of 3 cm or more, no abnormal morphological, beatiful color, and accepted by the market.
Novelty and actuality and scientific meaningfulness of the results

The project determined the optimum concentration of vitamin E and astaxanthin supplemented in broodstock diet which improved reproductive performance of clownfish.
The result of project also determined the Selco concentration in enriching live feeds, the appropriated time to change from rotifer diet to artemia nauplii and from artemia diet to commercial feed for the optimum growth and survial of fish A. ocellaris. 
In addition, the project also showed that canthaxanthin and  fructooligosaccharide concentration supplemented diet improved color, the growth of fish (in length, the specific growth rate in length and weight, the average weekly weight gain) and gut morphological characteristics of fish A. ocellaris. 

Products of the project
- Scientific papers in referred journals (list):
  • Effect of dietary vitamin E on reproductive performance, egg quality and larvae of clownfish Amphiprion ocellaris (Cuvier, 1830)
  • Effect of dietary fructooligosaccharide (FOS) on growth, survival and intestinal morphology of theocellaris clownfish, Amphiprion ocellaris Cuvier, 1830
  • Effects of dietary canthaxanthin on growth, survival, pigmentation and aesthetic of the ocellaris clownfish,Amphiprion ocellaris Cuvier, 1830
- Patents (list): Document accepted  the IP registration application with Intellectual Property Office Of Vietnam
- Patent name : THE PROTOCOL OF ARTIFICIAL BREEDING AND GROW-OUT CULTURE OF NEMO ANEMONEFISHES (Amphiprion ocellaris Cuvier, 1830).
- Technological products (describe in details: technical characteristics, place):The nemo anemonefish achieved a size of 3 cm or more, no abnormal morphological, beatiful color, and accepted by the market.
- Other products (if applicable):Training 01 Master of Science in Aquaculture 
Research region

VINA MARINEFARM TECHNOLOGY APPLICATION AND PRODUCTION CO., LTD – Address:  Khanh Nhon – Nhon Hai – Ninh Hai – Ninh Thuan province.