Project's information
Project's title | Developing the fluorescent image-based high content analysis towards screening of the anticancer compounds with regards to molecular targets and cellular models. |
Project’s code | VAST 04.05/18-19 |
Research hosting institution | Institute of Natural Products Chemistry |
Project leader’s name | Do Huu Nghi |
Job Title, The rank of a Professor, Academic degree | Dr. |
Project duration | 01/01/2018 - 31/12/2019 |
Project’s budget | 600 million VND |
Classify | Excellent |
Goal and objectives of the project | The main objective of this project aims to develop the image-based high content analysis (HCS/A) and then, create the standard operating procedures for screening of the anticancer compounds with regards to molecular targets and cellular models. |
Main results | - Scientific results: Two HCS/A-standard operating procedures for screening and bioactive evaluation of the anticancer compounds with regards to cellular models and molecular targets, e.g. cell-cycle arrest, apoptosis, protein translocation and mitochondria. - Applied results: Applied the HCS/A-based assays and analyzed by using an Olympus scanˆR station to screen some bioactive compounds for their anticancer activities with regards to molecular targets: + Cell-cycle arrest in phase G2/M of the Hep-G2 was observed for two samples Zer4 and CLV4; whilst SP13, Hw2, SCO1, HE11, VC1, and MurA inhibited the phase G0/G1. + Apoptosis-inducing activity: Hw2 sample was evaluated for its apoptosis-inducing activity by analysis of cell morphology as well as multi-channel fluorescence microscopy images (annexin V-FITC/PI) on Jurkat cell line that could be subsequently confirmed by a caspase-3/7 luminescent assay. + Zer4 was analyzed for inhibition of the NF-κB translocation from plasma into cell nuclear that monitored in living cells expressing a green fluorescent protein-tagged p65 (GFP-p65) subunit . In addition, cytotoxicity of some compounds against HeLa cell was also observed by applying the HCS/A analysis of mitochondrial target (MitoTracker® kit). |
Novelty and actuality and scientific meaningfulness of the results | To the author’s best knowledge, these are the first HCS/A-standard operating procedures to be domestically created toward screening of the anticancer activity with regards to molecular targets. |
Products of the project | - Scientific papers in referred journals (list): 02 paper in the national journal (Nghi D.H. et al. (2018) Application of image-based high content analysis for the screening of bioactive natural products. Vietnam J. Sci. Tech. 56: 1-12; Nghi D.H. (2020) Application of immunofluorescence in combination with high content imaging to screen the anti-translocation of nuclear factor NF-κB. Vietnam J. Biotech. Accepted). - Patents (list): 01 patent, application accepted No. 104726 / QD-SHTT, 22/11/2019 by the National Office of Intellectual Property. - Technological products (describe in details: technical characteristics, place): + 02 HCS/A-standard operating procedures for cell-based assays to screen the anticancer active compounds. + Reports of 08 anticancer agents with molecular targets. - Other products (if applicable): 01 MSc. and 02 students majoring in Biotechnology and Pharmacy. |
Research area | Academic institutions and university particularly in the fields of bioactive compounds, cellular biology, and pharmacology. |
Recommendations | Application of the HCS/A in screening of anticancer and anti-inflammatory activity; Continuously developing the HCS/A -based assays with regards to molecular targets to serve the bioactivity-guided chemical research. |