- Theoretical results:
+ Designing 3 expression vector constructions containing artifical H7 gene from H7 gene of H7N9 virus: pCB301/HA/ELP, pCB301/HApII/ELP, pCB301/HApII and generate Agrobacterium strains carrying the respective vectors.
+ Designing two help vector constructions containing pIBT/2bCMV, pIBT/Hc-Pro PRSV and generate Agrobacterium strains carrying the respective vectors.
+Expressing successfully HA/ELP, HApII/ELP and HApII protein using Agroinfiltration. Help vectors act well, improving transient expression of recombinant protein, especially Hc-Pro PRSV.
+Designing successfully transient expression procedure using pIBT/Hc-Pro PRSV: AS concentration is 400µM, OD600 is 0.5, leave’s position is middle, time after infiltration is 8 days
+ Purifying successfully HA-ELP and HApII-ELP using mITC complementing PEG 8000 with recovery rate of HA-ELP and HApII-ELP are 77.8 % and 78.5 %, respectively, purity of the HA protein / ELP is 91% and that of HApII / ELP is 95.4%. Purifying successfully HApII using IMAC with recovery rate as 64,6%, purity as 78%.
+ Successfully characterization of trimeric structure of HApII by Cross-linking reaction.
+ Successfully characterization of stucture of HA_pII:ND by measurement of size, Zeta potential and TEM. The size of ND increased 80nm after mixing with HApII protein. Zeta poteintial of NDs and HApII-ND is -45mV and -38 mV, respectively.
+ HA titer of HA/ELP, HApII-ELP and HApII is 0 HAU, 2 HAU, 2 HAU , respectively. Whereas HA titer of HApII:ND (1:12) is 1024 HAU (per 5µg of protein at the first well).
+ HApII/ELP, HApII and HApII:ND both elicited immune responses in mice, the best immune activity belongs to HApII:ND. At the 3rd immune response, the ability of protein eliciting immune responses is higher than the 2nd one.
- Applied results: HApII/ELP, HApII and HApII:ND elicit strong immune response in mice, which is potiental in development of vaccine against H7N9 virus. Some images of the project 
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