Project's information

Project's title Study on chemical constituents and hepatoprotective activity from root of Luvunga scandens (Roxb). Buch. Ham
Project leader’s name Dr. Mai Dinh Tri
Project duration 01/01/2015 - 01/01/2016
Project’s budget 600 millions vnđ
Classify Excellent
Goal and objectives of the project

- Study on the chemical constituents of root of Luvunga scandens (Roxb). Buch. Ham and hepatoprotective activity isolated compounds.
- Prepared preparations for in vivo hepatoprotective studies.

Main results

Theoretical results:
+ From the root of Luvunga scandens (Roxb). Buch. Ham, twenty two compounds, including one new limonoid, Luvunga A (17) and one new alkaloid, Luvungaside A (22), together with twenty known compounds: (E)-sinapinaldehyde (1), p-hydroxycinamaldehyde (2), protocatechuic aldehyde (3), protocatechuic acid (4), ostruthin (5), 6-geranyl-7-hydroxy-8-methoxycoumarin (6), bergapten (7), scopoletin (8), R-peucedanol (9), Xanthoarnol (10), acid umbelliferone-6-carboxylic (11), esculin (12), haploperoside (13), [(E)-3-(2,2-dimetyl-2H-chromen-6-yl) prop-2-enal (14), khellol glucoside (15), liomonin (16) và 3-O--D-glucopyranosylstigmast-5,22(E)-dien (18), 1,3-dihydroxy-2-methoxy-10-methyl-9-acridone (19), arborinin (20), 1-hydroxy-3-methoxy-10-methyl-9-acridone (21), were isolated by various chromatography methods. Among them, 6, 9, 10, 12, 13, 15 were found for the first time from the genus Luvunga, 1, 2, 3, 4,7, 8, 14 were reported for the first time from the species L. scandes.
+ The CHCl3, EtOAc, EtOH extracts and eleven compounds were tested their hepatoprotective effect and showed hepatoprotective activity against CCl4-induced hepatotoxicity on HepG2 cells (with prevention percentage of 20-195 %).
+ Preparations expressed hepatoprotective effect were constructed the extracted process.
+ Hepatoprotective activity in HepG2 human hepatoma cell line of preparations were evaluated by MTT assay. At the dose of 1.6g/kg decreased MDA, ALT, AST and increased GSH concentration significantly in comparision with extract-untreated group.
+ The HPLC-DAD method was validated for the qualitative and quantitative determination of major bioactive compound, arborinine. The result showed that content of arbornine in dried material and preparations were 0.011 and 0.096%, respectively.
+ Quality standards of preparations from this species were improved.

94.2017maidinhtri

94.2017maidinhtri1

94.2017maidinhtri2

Novelty and actuality and scientific meaningfulness of the results

For the first time, phytochemical investigation and hepatoprotective activity from root of Luvunga scandens (Roxb). Buch. Ham were studied. Two new compouds were isolated from this species.

Images of project
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