Project's information

Project's title Study on expressing recombinant sweet-tasting protein, Miraculin, in transgenic tomato plants
Research hosting institution Institute of Biotechnology
Project leader’s name As. Prof. Dr Chu Hoang Ha
Project duration 01/01/2013 - 01/01/2014
Project’s budget 500 million VND
Classify Excellent
Goal and objectives of the project

Creating transgenic tomato plants producing recombinant sweet-tasting protein, Miraculin, for food industry and pharmaceutical industry.
Main results

- Theoretical results:
 Codon optimization and artifical synthesis successfully of gene coding miraculin protein for tomatoes.
 Isolating and sequencing successfully E8 promoter from PT18 tomato, HSP18.2 promoter and HSP18.2 terminator from Arabidopsis and accessions in GenBank are KJ561284, KM083119 and KP008108, respectively.
 Designing three transgenic vector constructions containing mir gene, consist of pBI121/35S/mir/NOS, pBI121/E8/mir/NOS and pBI121/pHSP/mir/tHSP.
 Expressing successfully MIR protein in BY-2 tobacco cell, tobacco hair root and PT18 tomato.
- Applied results:
The mir transgenic tomato lines which have high accumulation of recombinant MIR protein are good materials to apply in food and phamaceutical industries.

35.2015chuhoangha

35.2015chuhoangha1
Novelty and actuality and scientific meaningfulness of the results

This is the first research in Vietnam for the expression of recombinant miraculin protein in plants and being also the first one in the world using tobacco BY-2 cells and hairs root cultural systems in order to the expression and biomass production miraculin protein. Therefore, this project has suggested new applications for making actively materials in industrial production.
Products of the project

- Scientific papers in referred journals: There are four papers
1. La Việt Hồng, Lê Hoàng Đức, Lê Văn Sơn, Phạm Bích Ngọc, Chu Hoàng Hà (2014) Cloning and analyzing of Cis-acting elaments in the E8 promoter from tomato (Lycopersicon esculentum L.) PT18. Journal of Biology 36(1): 118-124.
5. La Việt Hồng, Nguyễn Thu Giang, Phạm Bích Ngọc, Chu Hoàng Hà (2015) Expression of a taste-modifying protein , miraculin, in BY-2 tobacco cell line (Nicotiana tabacum L. Cv Bright Yellow 2). Journal of Biology 36 học 36 (3): 360-366. DOI: 10.15625/0866-7160/v36n3.5977.
2. La Việt Hồng, Phạm Bích Ngọc, Chu Hoàng Hà (2015) Cloning of HSP 18.2 promoter and terminator from Arabidopsis thaliana for construction of the gene expression vector in plant. Journal of Scienes, Vietnam national University, Hanoi. Accepted.
3. La Việt Hồng, Nguyễn Thu Giang, Phạm Thị Vân, Phạm Bích Ngọc, Chu Hoàng Hà. Enhancing expression of miraculin recombinant protein in tomato by selecting suitable promoter and terminator. Draft.
- Patents:
“The protocol of synthetizing, designing and expressing artificial miraculin protein in hair root cultural system”. Accepted.
- Technological products:

  • Artificial mir gene, optimizing codons.
  • Three nucleotide sequences, consist of promoter E8 (KJ561284), promoter HSP18.2 (KM083119) and terminator HSP18.2 (KP008108).
  • Three transgenic vector constructs, consist of pBI121/35S/mir/NOS, pBI121/E8/mir/NOS và pBI121/pHSP/mir/tHSP.
  • Three pBI121/E8/mir/NOS transgenic tomato lines and 2 chuyển gen pBI121/35S/mir/NOS transgenic tomato lines, containing one copy of mir gene in T0.