| Main results |
- Collected and inspected scientific names as well as created templates of 168 marine specimens of 04 branches: 61 corals, 60 algaes, 30 echinoderms samples and 17 mollusks samples. Analyzed composition, lipid content and fatty acids found in 168 samples, the results showed that the total lipid content of the samples are not high (0:51 to 2.32%). The composition and fatty acids content in these 168 samples are relatively uniform, mainly included fatty acids 16: 0, 18: 0, 20: 4n-6, 20: 5n-3. Evaluated the variation in total lipid content, the lipid class composition as well as the fatty acids composition in the total lipid of studied samples.
- The composition and lipid classes content in the total lipid of the researched samples contained sufficient basic classes of lipids, such as lipid polarization (PL), sterols (ST), free fatty acids (FFA), monoankyldiacylglycerol (MADG), triglycerol (TG), and wax ester (WE). The classes presented most in the hard coral are PL, TG and WE; PL, ST in soft corals; PL, ST, TG in echinoderms; PL, TG in algae and molluscs. The composition and fatty acids content in the class are mainly acids: 16: 0, 18: 0, 20: 4n-6, 20: 5n-3, 22: 6n-3.
- Determination on TLC was combined with standard substances in the same chromatographic conditions showed that the PGE2 signal only appear on these samples are three samples: HL-SHC 02, HL-SHM 03 and SHC 01 in the coral samples; HL-DG 04, HL-DG 02, HL-DG, HS 01, DG 15, DG 05 - Quao Ngọc and DG 01 - Quao Ngọc in echinoderms; the TM2, TM3, TM9 and TM13 samples in molluscs and Halophila ovalis (LP6, LP29), Padina australish (LP13), Polycavernosa fastigiata (LP17), Caulerpa verticillata (LP30), Gracilaria gigas (LP57, LP58), Gracilaria busas-pastoris (LP60). Analysis on GC and GC-MS: while analysing on GC and GC-MS, the appearance of PGE2 signal is only be seen in two samples Padina australish (LP13) and Polycavernosa fastigiata (LP17).
- The biological activity testing showed that: There are 150 of the total 176 samples (approximately 85.2% of the total samples) have activity against at least one of the 8 tested microorganisms. Therein, 62 samples have inhibitory activity to the (Gram +/-) bacteria’s development, 18 samples inhibiting the growth of fungi and no samples are active with all tested microorganisms. Samples LP 29 object seaweed algae, has active expression with the ability to neutralize free radicals generated by DPPH was SC%=54.98 ± 1.8% and SC50 = 37,69mg / ml. 50/176 samples have cytotoxic activity with at least one of the two tested cell lines. Therein, 20 samples have activity on the 2 liver cancer (Hep-G2) and lung cancer (LU-1) cell line.
- Results of isolating compounds: isolated 27 compounds from 04 research groups:
- Dichotella gemmacea species of Coral subject, has isolated 10 compounds which are: Cholesterol SHE.1.2, Praelolide, Juncelloside C, Juncelloside D, uracil, 1-glyceryl ether heptadecanyl, cholesterol, thymine, N-methyl-uracil, uracil SHE.3.5.4.13. Sample SHH 33 (Praelolide) has activity against S. aureus, MIC = 25μg / ml and activity against Hep-G2 cell line with IC50 = 7.58 g / ml and inhibited completely of the growth of these cells at a concentration of 10μg / ml.
- From Kappaphycus alvarezii and Gracilaria tenuistipitata species of subject seaweed algae, has isolated 06 compounds which are Methyl 2-hydroxyl tricosanoate, palmitic acid, 7β-hydroxy-cholesterol, 7α-hydroxy-cholesterol, 7β-methoxy-cholesterol, AAEE.
- From sea cucumber species Holothuria scabra and basket stars Archaster typicus of Echinodermata subject, we have isolated 11 compounds which are 4,14a-dimethyl-5a-cholest-9 (11) -en-3b-ol, lathosterol, thymine, phenyl alanine, holothurin B, TG1, TG2, TG3, TG4, TG5, Archasteroside C. Sample DGC 5.1 has antifungal activity (A. niger fungus, MIC = 25 g / ml; and yeast C . albicans, MIC = 50μg / ml and inhibitory activity of both hepatocellular carcinoma cell line (Hep-G2) and lung cancer (LU-1) with a 50% cells inhibitory concentration IC50 = 1,28μg / ml and IC50 = 1,34μg / ml, respectively. One strains resistant as DG 04.1 (B. subtilis resistant with MIC = 50μg value / ml) and the samples have activity against two kinds of bacteria are sample DGA 2.1 (B. subtilis resistance, MIC = 50μg / ml and S. aureus, MIC = 50μg / ml), E. coli resistant DG 4:10, MIC = 25μg / ml and B. subtilis, MIC = 50μg / ml)..
- From molluscs object, we has isolated 05 compounds: FA16: 0, 1-O-sn-glycerol-Octadecyl (18: 0), 1-O-tetradecyl-sn-glycerol (14: 0), EPA -ee, 22: 5n-3) DHA, 22: 6n-3.
- Semisynthetic simulated 24 eicosanoid compounds derived from marine organisms, serving for virtual research activities and correlate active-object structure on ER receptors. The results showed that the prostaglandin class has the potential inhibitors, cell cytotoxic higher than the Leukotriene class and Thromboxane class. Therein, Prostaglandin A1 and A2 are two compounds have the high potential cytotoxic activity. The quantum parameters and structure impact to varying degrees to active cytotoxic of researched eicosanoid molecules. QSAR model with parameters Et, Eb, H, μ, EHOMO, ELUMO, LogP and Eh best describes the virtual results (R2 = 0.6728) and can be used to predict activity cytotoxic of Eicosanoids compounds and orient for extraction and semi-synthesis.
- Successfully fabricated the micro emulsion containing curcumin nanoparticles, with curcumin concentrations about 5 - 25%; size particles 30-50 nm; zeta potential when dispersed in water is ~ -38 mV; have high stability at pH 7 for a long time (6-24 h), meeting the transport process requirements of the curcumin particles to desired destination. Antioxidant activity of curcumin was increased when curcumin in nano form and when curcumin combined with a synergistical substance belong to Flavonoid group as Quercetin.
- From the flesh of the Crassostrea gigas oyster after hydrolysis, we obtained high quality protein powder. Researched and Dosaged to determine the right recipe for Veda-K+ functional foods when capsulize 500 mg /capsule is N4 capsule formulation (protein hydrolysates 200 mg, Calcium stearate 150mg, acid ascosbic 10mg, Glucose 85mg, Curcumin 5g, Potato powder qs. Veda-K+ functional food does not show acute toxicity at dose studied levels, even max dose studied level 20g / KGP. Therefore, the value LD50 (lethal dose 50% of laboratory animals) is not determined, thus suggesting that Veda-K+ functional foods in form of capsule does not cause acute toxic and non-lethal to mice. Veda-K+ functional food in tablets form did not affect the increase of weight of tested animals despite being given in high doses extends continuously. Beside, functional food Veda-K+ in tablets form does not affect tissues and organs in the body, does not affect the physiological function of the organ in the body.
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