Project's information

Project's title Using ADN sequencing to support the traditional morphological taxonomic identification of species of bamboos (Bambusa Schreb.)
Project leader’s name Prof. Dr. Dinh Thi Phong
Project duration 01/01/2011 - 01/01/2012
Project’s budget 770 Million VND
Classify Excellent
Goal and objectives of the project

Using methods of ADN analysis to support traditional morphological taxonomic ones in identifying accurately  some Bamboo species (Bambusa Schreb) which are valuable resources in Vietnam, for their storaging and displaying in VNMN.

Main results

Theoretical results: Through the decode of the nucleotide sequences of the 04 gene regions for 06 bamboo species in Vietnam, we concluded that  03 species needed revisions on the genus name, 03 species needed further analysis as their differences might be traced back to morphological changes and identify 05 gene regions for 08 bamboo species. The results  confirmed the effectiveness of ADN analysis methods in the classification of this complex taxa  whose many issues still need to be studied. Moreover, these results proved to be an useful tools for Vietnamese botanists in identifying the scientific names for two bamboo species B. vulgaris Schrader ex Wendland cv. vittata McClure and B. vulgaris Schrader ex Wendland.cv. wamin McClure; as the research suggested that they might be B. tuldoides and three other bamboo species, named [Bambusa (Lingnania) sp.], [Bambusa (Lingnania) chungii] and [Bambusa (Lingnania) lougissima sp. nov.] of the Bambusa genus;

  • Among the 08 species of bamboo needed identification, the so-called tan an bamboo was identified as a new bamboo species of the Vietnam coulded be named Bambusa Tananesis.
  • During our study, we registered 57 specific ADN sequences of bamboo species in the Genbank with coded from JX428387 - JX428443. We published 04 articles, trained 02 MAs and built a collection of 14 bamboo species, which is currently kept at the Museum of Nature Vietnam.

Applied results included: The contribution to bamboo species identification using ADN technology independently from traditional morphological taxonomic methods; along with the protection of national sovereignty for Vietnam's endemic species of bamboo.

Novelty and actuality and scientific meaningfulness of the results
  • Using ADN analysis methods to support the traditional morphological classification method for some of bamboo species (Bambusa Scherb.) for the first time in Vietnam
  • By decoding the results of 4 chloroplast gene regions and 01 nuclear gene region, we came to the conclusion that: (1) Three species, so-called [Bambusa (Lingnania) sp.], [Bambusa (Lingnania) chungii] and [Bambusa (Lingnania) longissima] in the past are confirmed to belong to the Bambusa genus: (2) Bambusa vulgaris Schrader ex Wendland cv. vittata McClure and B. vulgaris Schrader ex Wendland.cv. wamin McClure were synonyms of B. tuldoides and both of them were only cultivars (cv.); (3) the commonly known Tan an bamboo  was identified a new species of Vietnam.
Recommendations

We request Vietnam Academy of Science and Technology of Vietnam continued the funding for the project to aid our process of decoding some regions of the gene to help classify the 7 unidentified bamboo species (currently named as Bambusa sp.), whose results, in the long term, will be the basis for the classification of species belonging to the Bambusa genus.