Project's information

Project's title Evaluation of gene expression at mRNA level of candidate genes assisted to diagnostic breast cancer by real time RT-PCR
Research hosting institution Institute of Biotechnology
Project leader’s name Dr Nguyen Thi Dieu Thuy
Project duration 01/01/2009 - 01/01/2010
Project’s budget 300 000 000 VND
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Goal and objectives of the project

Optimization of real time RT-PCR to determine gene expression at mRNA level of mammaglobin (MAM) and carcinoembryonic antigen (CEA) genes in breast cancer patients.
Main results

Theoretical results:

- Sample collection (blood-tissue) for gene expression analysis of breast cancer.

- Total RNA samples from blood and tissue from normal tissue/blood and cancerous turmor of breasr cancer.

- Optimization of real-time RT-PCR conditions (concentration of primers, probes, thermal cycle, PCR parameters) evaluation of relative gene expression of MAM and CEA at mRNA level.

- Relative gene expression of MAM and CEA at mRNA level in tissue and blood samples.

* Blood sample:
+ No significant differences at mRNA expression level of MAM/CEA were found in blood samples of normal and breast cancer patients.

* Tissue sample:
+ Gene expressions of MAM at cancerous turmo were higher 1.43-4.69 (single gene) and 3.65-18.88 (dual genes) than that of normal tissue of breast cancer patients.
+ Gene expressions of CEA at cancerous turmo were higher 1.93-4.72 (single gene) and 1.68-13.11 (dual genes) than that of normal tissue of breast cancer patients.
Novelty and actuality and scientific meaningfulness of the results

Real-time RT-PCR was used for evaluation of gene expression at mRNA level of Mammaglobin and Carcinoembryonic antigen genes at breast cancer patients in Vietnam.
Products of the project

- Scientific papers in referred journals (list):

  • Nguyen Thi Dieu Thuy, Ta Thi Loan, Nguyen Van Cuong, Le Thi Phuong, Nguyen Thi Binh, Le Hong Quang, Nguyen Van Dinh, Ta Van To. 2010. Expression level of mammaglobin gene in breast cancer patients. Journal of Practical Medicine, No. 745 (Dec 2010): 92-94.
  • Le Thi Phuong, Ta Van To, Nguyen Thi Dieu Thuy et al., 2010. Remark about some clinical and histopathologic features of women with positive estrogen and progesterone receptors at Hospital-K. Viet Nam Journal of Medicine, No. 1/2010:17-22.

- Technological products (describe in details: technical characteristics, place):

  • Blood/tissue/turmo sample set for analysis of breast cancer.
  • Real-time RT-PCR method for evaluation of relative gene expression of Mammaglobin and Carcinoembryonic antigen genes in breast cancer patients.