Project's information

Project's title MASSREAING BEAN APHIDS Aphis craccivora Koch (APHIDIDAE) FOOD PROCESSING AND DISTRIBUTION ARTIFICIAL REARING SPODOPTERA LITURA (NOCTUIDAE) TO ADOPT A FEED INSECTS FEEDINGS USED IN BIOLOGY CONTROL PEST
Project leader’s name Dr. Truong Xuan Lam
Project duration 01/01/2010 - 01/01/2011
Project’s budget 30 000 000 VND
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Goal and objectives of the project

To improve breeding techniques bean aphid Aphis craccivora (Aphididae) and blending foods deep cavity artificial rearing Spodoptera litura (Noctuidae) in the laboratory to produce food in large quantities, initiative and clean alternative to passive source of food, unsafe collected in the wild for breeding several species of insect prey for the research, experimental biology and fight pests in plants.

Main results

Use absorbent cotton sterile as the bed to bean grows in labroom. The stainless steel tray which we washed, dried and dried in a 80oC temperature for 30 minutes to clean the spores (fungi ...) start to damage the beans when they when beans are sown or started to germinate. The next step is a stainless steel ball on the tray with the appropriate thickness. After finished the spread out wet cotton in hydroponics containing additional service to the standards of the roll out. Beans are selected when the pea plant varieties (seeds, machinery, were not wrinkled skin, rough or scratched ..), Put beans in warm water, around 50oC (approximately equal to 2 + 3 cold boiled water). Processing time was a bean in warm water 10-15 minutes, then we pour out, drain and dry on a tray carried a price sowing may have been wet by the solution.
+ Rearing aphids feed on the black beans grown in hydroponic solution can efficiently produce a number of aphids in the laboratory conditions, after 7-10 days can be obtained 10.000 -15.000 aphids / tray culture.
- Spread on the tray in a layer about 3 mm thick, cotton cover is 15g per tray.
- Irrigation hydroponic solution to wet all cotton, 300ml/tray
- Black beans treated with warm water 55°C in 7-10 minutes, sprinkle on the surface layer of cotton, beans 100g/khay amount.
paper coated with a surface parking, with your hands to pat the paper layer is absorbed hydroponic solution and squeeze it on cotton and beans. Each roll is 10 the tray cover.
The trays were sown beans and cover paper in a culture chamber set at temperature 28°C, humidity 75-85%.
- Implant seed bug: After 3 days cultivation, pea seed germination. When sprouts 1 cm high, starting transplants like aphids. Use scissors to cut the tops sprinkle with bean aphids on the new bean tray, each tray has about 20-30 tops bean aphids. Bugs will crawl from the flame to pass new bean sprouts and continue to reproduce. Every day more irrigation hydroponic solution to the tray.
Crops: After 70-10 days culture aphids were able to collect bugs ladybugs feed. For every 100g of black bean seed sown tray estimated aphids collected in each tray can reach 10.000-15.000 aphids, raising enough larvae 100-150 / day.
+ Blending techniques feed deep cavity completely and can be applied in the laboratory to produce deep cavity with survival in F1 and F2 are very high up> 90% and 90% pupation rate.
             Raising deep cavity with artificial food, they complete the life cycle and with the indicators mentioned above as the survival rate is very high in F1 and F2 accounted for over 90% pupation rate were 90%. And all the biological criteria to monitor the F1 generation is the highest development time of larvae and pupae shorter than F2 and F3. The rationale for this part due to degeneration in the same culture across generations as well as the effect of raising the room temperature in humidity differ across generations, possibly due to an artificial diet is meet the needs of all the essential nutrients for life processes, chemical weapons, body weight, development time of larvae, pupae and number of eggs laid. But with this result we can confirm, the prototype feed deep cavity with the process and the formula was effective in raising deep cavity, one of the following sources to meet the breeding different species of natural enemies in the laboratory.
+ Ability to eat some insects feeding (feeding ladybugs, bugs prey and prey search plants) by breeding food has met the criteria for laboratory experiments and high results + Human aphids feed on the crop black beans black beans grown in hydroponic solution can efficiently produce a number of aphids in the laboratory conditions, after 7-10 days can be obtained 10 000 -15 000 aphids / tray culture.
- Ball on the tray in a layer about 3 mm thick, cotton cover is 15 g per tray.

Research region
  • Experimental Entomology Department
  • Insect Ecology Division - Institute of Ecology and Biological Resources
  • Office-Plant Protection Plant Protection Centre in the North-Plant Protection Department
  • The agency tested pesticides.