Project's information

Project's title Study the effects of nano ceria on the growth, development, physiology and biochemistry of Chrysanthemum and Fragaria × ananassa plantlets cultured in vitro
Project’s code THTETN.10/24-25
Research hosting institution Institute of Life Science
Project leader’s name Dr. Do Manh Cuong
Project duration 01/01/2024 - 30/06/2025
Project’s budget 500 million VND
Classify Grade B
Goal and objectives of the project

Providing scientific data on the effects of nano ceria on the growth, development, physiology and biochemistry of Chrysanthemum and Fragaria × ananassa plantlets cultured in vitro

Main results
- Theoretical results: Some new scientific information is initially provided on the effects of CeO2NPs on the growth, development, nutrient absorption, and antioxidant enzyme activity of Chrysanthemum and Fragaria × ananassa plantlets cultured in vitro for research and teaching activities.
- Applied results: The project's results have high potential for application in the field of plant micropropagation
Novelty and actuality and scientific meaningfulness of the results
- Fragaria × ananassa: The medium supplemented with 1.0 mg/L SiO2NPs showed enhanced mineral absorption, while the medium supplemented with 1.0 – 1.5 mg/L CeO2NPs created the most favorable conditions for the activity of antioxidant enzymes. As a result, the plantlets at these concentrations also exhibited superior morphological and physical characteristics. The creative combination of SiO2NPs and CeO2NPs in RSM-based culture medium has great scientific and practical significance in Fragaria × ananassa plantlets in vitro propagation, not only maximizing plant growth but also overcoming the weakness of stems.
- Chrysanthemum: The addition of CeO2NPs under salt stress conditions based on RSM increased the free radical scavenging activities of antioxidant enzymes (SOD, CAT, APX). In this study, the maximum salt tolerance threshold of Chrysanthemum and the corresponding CeO2NPs concentration were 30 mM NaCl and 12.8 mM CeO2NPs. Under these culture conditions, the adaptability of in vitro plantlets to salt stress condition was enhanced, leading to the improvement in morphological and physiological characteristics, thereby increasing crop yield under salinity stress.
Products of the project
- Report: 01
Evaluation of the effects of nano ceria on the growth, development, physiology and biochemistry of chrysanthemum and strawberry plants cultured in vitro
- Scientific papers in referred journals (list): 02
Cuong DM, Mai NTN, Luan VQ, Tung HT, Thuy NTT, Nguyen PLH, Hiep PPM, Dang HH, Hoang CV, Vinh NQ, Nhut DT (2024) Optimizing the combination of SiO2NPs and CeO2NPs on relative growth rate of Fragaria× ananassa plantlets cultured in vitro. Plant Cell Tiss Org 159: doi:https://doi.org/10.1007/s11240-024-02902-6
Cuong DM, Mai NTN, Luan VQ, Hiep PPM, Dang HH, Vinh BVT, Hoang CV, Tung HT, Vinh NQ, Nhut DT (2025) Enhancing antioxidant enzyme activities in chrysanthemum plantlets (Chrysanthemum morifolium Ramat cv. 'Jimba') under salt stress: A response surface methodology approach evaluating NaCl and CeO2 nanoparticles interactions. Plant Cell Tiss Org 161: doi https://doi.org/10.1007/s11240-025-03096-1
Recommendations

- From the above results, we propose to conduct further researches on the action mechanism of other rare earth nanoparticles to optimize the application of nanotechnology in plant cell technology.
- In addition, it is necessary to expand the application of nano in other areas such as protecting plants from diseases and improving the resistance of plants to extreme environmental conditions. At the same time, research and assessment of the safety and long-term impact of nanoparticles on the environment and human health are very important to ensure the sustainability of nanotechnology applications.
- We respectfully request that the Vietnam Academy of Science and Technology continue to provide financial support for the research group to continue the research orientation on nanotechnology in the field of plant cell physiology.

Images of project
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