1) To enhance capacity on research and apply of the key-labs “Food and Environment safety” established by Vietnam Academy of Science and Techlology in order to protect human health, customer ’s right for substainable development. 
2) To develop scientific research and technology on seafood safety and produce nesscessary products for research and taking care of human health. 
Objectives:
1) To develop practical protocols to identify toxins and toxic substances in seafood in order to evaluate seafood quality, the risk to human health and safety limit. 
2) To identify toxic substances and their mechanism to accumulate, dispatch and evaluate seafood quality effected by human activities.
3) To develop the practical protocols for reservation of seafood.
4) To develop and apply the protocols to identify and analyse toxic substances in commercial seafood product.
5) To create data on chemical profile of some seafood products in Viet Nam.
6) To obtain reference materials for research and testing toxins in seafood.

- The project, for the 1st time to develop successfully the comprehensive practical protocol to identify and evaluate toxins and toxic substances in marine environment, in order to complete and enhance research capacity in VAST and Vietnam.
- Two reference materials have been developed to surve analytical condition of Vietnam. 
- The important scientific results of the project are new finding in Viet Nam and in the world, have been publised on SCIE journal to affirm Vietnamese scientific ability. 

- Scientific papers in referred journals: 12 SCIE articles, 13 national articles, and 02 patent applications.
SCIE papers:
1. Ha Viet Dao, Aya Uesugi, Hajime Uchida, Ryuichi Watanabe, Ryoji Matsushima, Zhen Fei Lim, Steffiana J Jipanin, Ky Xuan Pham, Minh-Thu Phan, Chui Pin Leaw, Po Teen Lim, Toshiyuki Suzuki (2021). Identification of Fish Species and Toxins Implicated in a Snapper Food Poisoning Event in Sabah, Malaysia, 2017. Toxins 2021, 13, 657. https://doi.org/10.3390/toxins13090657. (ISSN: 2072-6651; SCIE; IF: 4.2; Q1).
2. Ha Viet Dao, Hy Ho Khanh Le, Thao Thi Thu Le, Ky Xuan Pham, Minh Quang Bui & Leo Lai Chan (2022). Ciguatoxin in moray eels raising the risk for seafood safety in Viet Nam. Fisheries Science 88, 821-830. https://doi.org/10.1007/s12562-022-01638-6. (e.ISSN:1444-2906; Print ISSN:0919-9268; IF: 1.9; Q2)
3. Dang Thuan Tran, Cam Van T. Do, Cuc T. Dinh, Mai T. Dang, Khanh Hy Le Ho, Truong Giang Le and Viet Ha Dao (2023). Recovery of tetrodotoxin from pufferfish viscera extract by amine-functionalized magnetic nanocomposites, RSC Advances, 13, 18108–18121 (SCI-E, IF= 4.036, Q2);
4. Dang Thuan Tran, Cuc T. Dinh, Mai T. Dang, Cam Van T. Do, Khanh Hy Le Ho, Truong Giang Le, Viet Ha Dao (2023). Central composite design-based response surface methodology for optimization of tetrodotoxin extraction from the horseshoe crab Carcinoscorpius rotundicauda. Asia-Pac Journal of Chemical Engineering, e2974 (SCI-E, IF= 1.8, Q3). doi:10.1002/apj.2974;
5. Nguyen XV, Phan TTH, Cao VL, Nguyen-Nhat NT, Nguyen TH, Nguyen XT, Lau VK, Hoang CT, Nguyen TMN, Nguyen MH, Dao VH, Teichberg M, Papenbrock J. 2022. Current advances in seagrass research: A review from Viet Nam. Frontiers in Plant Science. 13: 991865. doi: 10.3389/fpls.2022.991865
6. Nguyen XV, Liao ML, and Lin SM. 2023. Systematic revision of the Grateloupia complex (Rhodophyta) from Vietnam using rbcL and LSU sequences, with the description of Phyllymenia nhatrangensis sp. nov. Phycologia 62(5), 436-444. doi: 10.1080/00318884.2023.2247175.
7. Xuan Ben Hoang, Minh Quang Thai, Phan Minh-Thu, Xuan Ky Pham, Nguyen Ngoc Tung, and Ha Viet Dao (2022). Antipredator Defenses in Soft Corals of the Genus Sarcophyton (Octocorallia; Alcyoniidae) from Coastal Waters of Central Vietnam. Russian Journal of Marine Biology 48(2), 122–128. DOI: 10.1134/S1063074022020055.
9. Tran-Lam, T. T., Quan, T. C., Pham, P. T., Phung, A. T. T., Bui, M. Q., & Dao, Y. H. (2023). Occurrence, distribution, and risk assessment of halogenated organic pollutants (HOPs) in marine fish muscle: The case study of Vietnam. Marine Pollution Bulletin, 192, 114986.
9. Tran-Lam, T. T., Phung, A. T. T., Pham, P. T., Bui, M. Q., Dao, Y. H., & Le, G. T. (2023). Occurrence, biomagnification, and risk assessment of parabens and their metabolites in marine fish: The case study of Vietnam. Chemosphere, 344, 140221.
10. Tran-Lam, T. T., Quan, T. C., Bui, M. Q., Dao, Y. H. & Le, G. T. (2024). Endocrine-disrupting chemicals in Vietnamese marine fish: Occurrence, distribution, and risk assessment . Science of the Total Environment 908. 168305. https://doi.org/10.1016/j.scitotenv.2023.168305.
11. Vu Duc, N., Le Minh, T., Nguyen, H. T., Dam, D. A., Nguyen Thi, X., Chu, D. B., Hoi, B.V, Hoang, A.Q., Manh, T.T., Pham, T. N. M. (2024). Halogenated organic pollutants (HOPs) in marine fish collected from central-northern coastal waters in Vietnam. International Journal of Environmental Analytical Chemistry, 1–20. https://doi.org/10.1080/03067319.2023.2299740
12. Vu-Duc, N., Duong-Thi, Q. M., Bui, V. H., Vu-Cam, T., Nguyen, T. T., Nguyen, T. T., Le, N-T., Nguyen, H.T., Nguyen, H.A., Chu, D. B. (2023). Analysis of perfluoroalkyl compounds in marine fish muscle samples by liquid chromatography tandem mass spectrometry. International Journal of Environmental Analytical Chemistry, 1–17. https://doi.org/10.1080/03067319.2023.2285377
National papers
1. Dao Viet Ha, Le Ho Khanh Hy, Pham Xuan Ky, Bui Quang Nghi, Nguyen Phuong Anh, Phan Bao Vy, Doan Thi Thiet (2023). Frequent occurrence of tetrodotoxin in the marine gastropod Nassarius glans causing a food poisoning in Khanh Hoa province, Vietnam in 2020. Vietnam Journal of Marine Science and Technology 23(2), 203–208. https://doi.org/10.15625/1859-3097/18080. (ISSN 1859-3097; e-ISSN 2815-5904).
2. Pham Thi Mien, Jutta Wiese, Dao Viet Ha (2023). Metabolites from the soft coral -associated bacterium Micrococcus sp. strain A-2-28. Vietnam Journal of Marine Science and Technology 23(2), 181–188. https://doi.org/10.15625/1859-3097/18414 (ISSN 1859-3097; e-ISSN 2815-5904).
3. Pham Thi Mien, Dao Viet Ha, Cornelis Verduyn (2023). Nutritional effects on biomass and metabolic products by Aurantiochytrium sp. Vietnam Journal of Science, Technology and Engineering, Part C. 65(4). 94-100. DOI: 10.31276/VJSTE.65(4).94-100.
4. Dao Viet Ha, Le Ho Khanh Hy, Pham Xuan Ky, Nguyen Ngoc Tung, Hajime Uchida (2021). Identification of Ciguatoxin-1b using LC/MS-MS with Selected Ion Monitoring (SIM) technoque in Vietnam. The Proceeding on The 7th analytica Vietnam Conference. Ha Noi. ISBN: 978-604-369-618-9.
5. Do Thi Cam Van, Nguyen Thi Phuong Dung, Tran Dang Thuan (2023) (in Vietnamese). Phát triển qui trình chiết xuất nâng cấp để thu hồi tetrodotoxin từ nội tạng cá nóc. Tạp chí Khoa học & Công nghệ Trường Đại học Công nghiệp Hà Nội, Vol. 59 - No. 6B, trang 128-134. http://hdl.handle.net/123456789/2741.
6. Dinh Thi Cuc, Dang Thi Mai, Do Thi Cam Van, Tran Huu Quang, Tran Dang Thuan (2023). Seasonal, body weight and gender variation of tetrodotoxin in horseshoe crab Carcinoscorpius rotundicauda collected from Can Gio coast, Ho Chi Minh city, Vietnam. Tạp chí Khoa học Trường Đại học Quy Nhơn, 7(5), 61-73. DOI: https://doi.org/10.52111/qnjs.2023.17506.
7. Nguyen XV, Nguyen-Nhat NT, Nguyen XT, Le-Ho KH, Pham DT, Dao VH. 2023. Effect of environmental parameters on the content and sugar composition of sulfated polysaccharides in some tropical seagrass. Vietnam Journal of Marine Science and Technology 23(3), 321–330. doi: 10.15625/1859-3097/18053.
8. Doan Thi Thiet, Pham Xuan Ky, Dao Viet Ha, Nguyen Phương Anh, Le Ho Khanh Hy, Phan Bao Vy (2023). Investigate lipid and protein oxidation in herring (Sardinella gibbosa) by sodium acetate during preservative time. Vietnam Journal of Marine Science and Technology 23(3) 293–301. https://doi.org/10.15625/1859-3097/17612.
9. Phan Bao Vy, Le Ho Khanh Hy, Nguyen Phuong Anh, Doan Thi Thiet, Pham Xuan Ky, Dao Viet Ha (2023). Investigation of antioxidant activity of Zoanthus vietnamensis’s crude extract and dichloromethane extract. Vietnam Journal of Marine Science and Technology 23(4) 419–427. https://doi.org/10.15625/1859-3097/18293
10. Thuy, Q. C., Phuong, P. T., Thien, T. L. T., & Minh, B. Q. (2022). Determination of seven parabens in surface water samples by UHPLC‐MS/MS and solid‐phase extraction. Vietnam Journal of Chemistry, 60(6), 738-743.
11. Bui Quang Minh, Phung Thi Anh Tuyet, Pham Thi Phuong, Dao Hai Yen (2023). Method development for the determination of organophosphate ester flame retardants in fish samples by modified QuEChERS followed by GC-MS/MS. Tạp chí Phân tích Hóa, Lý và Sinh học 29(03).135-141.
12. Pham Thi Diem Quynh,  Pham Thi Ngoc Mai,  Hoang Quoc Anh,  Le Minh Thuy,  Nguyen Thi Huong,  Vu Khanh Hoa,  Do Thi Thu Huong,  Nguyen Thi Xuyen,  Nguyen Xuan Hung,  Chu Dinh Binh,  Vu Duc Nam (2023). Determination of polychlorinated biphenyls in marine fish samples by gas chromatography tandem mass spectrometry (GC-MS/MS). Vietnam Journal of Food Control 6(2), 126-142. DOI: https://doi.org/10.47866/2615-9252/vjfc.4072.
13. Le Minh Thuy, Dam Duc Anh, Nguyen Thi Huong, Vu Khanh Hoa, Do Thi Thu Huong, Pham Thi Loan Ha, Nguyen Thi Thu Huyen, Chu Dinh Binh, Vu Duc Nam (2023). Study on simultaneous determination of OCPs, PCBs and PBDEs in fish sample: application for marine fish tissues collected from Hai Phong province. Vietnam Journal of Food Control 6(4), 366-379, DOI: https://doi.org/10.47866/2615-9252/vjfc.4158.
- Patents (list) (In Vietnamese)
1. PHƯƠNG PHÁP CHẾ TẠO VẬT LIỆU NANO TỪ TÍNH IN DẤU PHÂN TỬ TETRODOTOXIN (accepted a valid application by the National Office of Intellectual Property on 29/08/2023).
2. QUY TRÌNH TINH CHẾ TETRODOTOXIN TỪ NỘI TẠNG CÁ NÓC DÙNG VẬT LIỆU NANO TỪ TÍNH IN DẤU PHÂN TỬ TETRODOTOXIN (accepted a valid application by the National Office of Intellectual Property on 29/08/2023).
- Technological products (describe in details: technical characteristics, place):
+ 06 practical protocols for quantitative analysis of natural toxins from marine fauna in Vietnam: 
* Protocol of quantitative analysis of tetrodotoxins in marine fish in Vietnam using HPLC-MS LCMS 8040 Shimadzu;
* Protocol of quantitative analysis of tetrodotoxins in marine molluscs in Vietnam using HPLC-FD Shimadzu 20AD;
* Protocol of quantitative analysis of tetrodotoxins in marine crustaceans in Vietnam using HPLC-FD Shimadzu 20AD;
* Protocol of quantitative analysis of saxitoxin in marine crustaceans in Vietnam using HPLC-FD Shimadzu 20AD and post-column reaction system;
* Protocol of quantitative analysis of domoic acid in marine bivalve in Vietnam using HPLC-UV Shimadzu 20 AC;
* Protocol of quantitative analysis of ciguatoxin in reef fish in Vietnam using LCMS 8040 Shimadzu;
+ 06 practical protocols for extracting natural toxins from marine fauna in Vietnam: 
* Protocol of extracting tetrodotoxin from some puffer fish species in Vietnam
* Protocol of extracting tetrodotoxin from marine molluscs in Vietnam
* Protocol of extracting tetrodotoxin from marine crustaceans in Vietnam
* Protocol of extracting domoic acid from marine bivalve in Vietnam
* Protocol of extracting saxitoxin from marine crustaceans in Vietnam
* Protocol of extracting ciguatoxin from coral reef fish in Vietnam
+ Protocol for purification of TTX from pufferfish viscera TTXs-rich extract using Fe3O4@SiO2-TTXMIP to TTX purity of 95.7%
+ protocol for purification of STX from Carcinoscorpius rotundicauda STXs-rich extract using Fe3O4@SiO2-STXMIP to STX purity of 95.0%
+ Set of standard quantitative analysis protocols of toxics in seafood (17 protocols):
* Analytical protocol of toxic metals in marine foods (As) employing ICP – MS
* Analytical protocol of toxic metals in marine foods (Hg) employing ICP – MS
* Analytical protocol of toxic metals in marine foods (Pb) employing ICP – MS
* Simultaneous analytical protocol of toxic metals in marine foods (As, Hg, Pb) employing ICP – MS
* Analytical protocol of toxic metals in marine foods (Se) employing ICP – MS
* Analytical protocol of metal speciation (inorganic and organic forms) in marine foods (Hg) employing ICP – MS and GC - MS
* Analytical protocol of metal speciation (inorganic and organic forms) in marine foods (Pb) employing ICP – MS
* Analytical protocol of metal speciation (inorganic and organic forms) in marine foods (As)
* Simultaneous analytical protocol of metal speciation (inorganic and organic forms) in marine foods (Hg and Pb)
* Analytical protocol of pesticide residues in marine foods (OPPs)
* Analytical protocol of pesticide residues in marine foods (OCPs) employing Gas Chromatography Tandem Mass Spectrometry (GC-MS/MS)
* Simultaneous analytical protocol of pesticide residues in marine foods (OCPs, PCBs) employing Gas Chromatography Tandem Mass Spectrometry (GC-MS/MS)
* Simultaneous analytical protocol of pesticide residues in marine foods (OCPs, PCBs, PBDEs) employing Gas Chromatography Tandem Mass Spectrometry (GC-MS/MS)
* Analytical protocol of organic toxins in marine foods (PAHs) employing Gas Chromatography Tandem Mass Spectrometry (GC-MS/MS)
* Analytical protocol of organic toxins in marine foods (PBDEs) employing Gas Chromatography Tandem Mass Spectrometry (GC-MS/MS)
* Analytical protocol of organic toxins in marine foods (PCBs) employing Gas Chromatography Tandem Mass Spectrometry (GC-MS/MS)
* Analytical protocol of organic toxins in marine foods (PCPs) employing High performance liquid chromatography (HPLC)
+ 03 protocols of toxic identification, sample processing, data processing for fish samples:
* Analytical protocol of chlorophenols in fish
* Analytical protocol of flame retardants (FRs) in fish
* Analytical protocol of Endocrine-disrupting chemicals (EDCs) in fish
+ 04 Protocols of preservation for seafood safely and effectively:
* Protocol of preservation for skipjack tuna after catching;
* Protocol of preservation for herring after catching;
* Protocol of preservation for squid after catching;
* Protocol of preservation for blue crab after catching;
+ 01 set of VILAS profile for expanding ISO/IEC 17025:2017 registration for analytical indicators of metals (As, Hg, Pb) and other highly toxic organic compounds;
+ Specimen collection of toxic marine animals (fish, mollusc, crustacea) in the Musium of Institute of Oceanography. 
+ Reference materials 2 mg STX and 2 mg TTX (purity 95%) in Institute of Chemistry.
+ General Scientic Report.
+ Other products: educated 08 masters and supported training of 01 doctorate student.

The scientific results of the Project will be the scientific basis for fields of study related to toxins in marine organisms in particular, and marine science in general at institutions, universities inside and outside the Vietnam Academy of Science and Technology, and local units in coastal provinces of Vietnam.