Project's information

Project's title Study of oil polluted treatment technology at sea by bioremediation
Project’s code CP1862.02/20-22
Research hosting institution Institute of Biotechnology
Project leader’s name Kieu Thi Quynh Hoa
Project duration 01/05/2020 - 31/12/2023
Project’s budget 15,000 million VND
Classify Grade B
Goal and objectives of the project
Main Goals and objectives of the project
Develop a process for oil polluted treatment by Bioremediation in coastal tidal areas, using microorganisms capable of oil degradation and supplementing appropriate nutrients to improving treatment efficiency and ensuring ecological safety.
Specific Goals and objectives of the project
- Develop a technological process for producing biological products to degrade oil and create biosurfactants from microorganisms to apply in treating oil-contaminated sand/sediment in coastal tidal areas.
- Develop a technological process for treating oil pollution in coastal tidal areas using the bioremediation method.
- Establish a demonstration model for treating oil pollution in coastal tidal areas using the bioremediation method.
Main results
- Theoretical results:
1. Environmental parameters and some groups of microorganisms were surveyed in the model building area
2. Complete the collection and maintain the activity of two mixtures of microbial strains (bacteria and yeast) that have capable of degrading oil and synthesizing biosurfactants. 
3. Production process of biological oil-degrading products from bacteria and yeasts for application in treating oil pollution in coastal intertidal zone (supratidal and intertidal zones) was developed.
4. Process of treating oil pollution in coastal tidal zones (supratidal and intertidal zones) by bioremediation method was developed.
5. The effectiveness of using oil-degrading products from microorganisms was evaluated.
Applied results:
1. Production process of biological oil-degrading products from bacteria and yeasts for application in treating oil pollution in coastal intertidal zone (supratidal and intertidal zones)
2. Successfully built and operated models (21m2) to treat oil pollution in coastal tidal areas using bioremediation method in summer and winter.
3. Successfully built and operated models (200m2) to treat oil pollution in coastal tidal areas using bioremediation method in summer and winter.
Novelty and actuality and scientific meaningfulness of the results

After drifting ashore, oil often concentrates above the intertidal zone and then combines with the surface layers (sand or gravel) of the beach (above the lowest water level at low tide and below the highest water level at supratidal zone). Therefore, in addition to complete the technological process and model for oil-contaminated treatment in the supratidal zone, we are building a technological process and model for oil-contaminated treatment in the intertidal zone using the Bioremediation method. The construction of models in the intertidal zone to propose a treatment process is necessary and is a new point of the project, which is being carried out for the first time in Vietnam. The success of this task will contribute significantly to the field of applying research results for oil-contaminated treatment in coastal zone by bioremediation method to reduce environmental pollution and protect public health in coastal areas affected by oil spills.

Products of the project

- Scientific papers in referred journals (list):
* 02 international puplications (ISI)
1. Nguyen Huu Dat, Vu Manh Tuan, Mai Duc Huynh, Tran Hưu Trung, Nguyen Thi Thu Trang, Dam Xuan Thăng, Kieu Thi Quynh Hoa and Nguyen Vu Giang (2022). Novel Method for producing oleophilic polyurethane foam to remove oil from open water. Journal of Polymers and the Environment. 30:5012-5023.
2. Huynh Mai Duc, Dat Nguyen Hưu, Trung Tran Huu, Lu Le Trong, Hai Lương Nhu, Hong Phan Ngoc, Thao Nguyen Van, Quynh Hoa Kieu Thi and Giang Nguyen Vu (2021). The effect of Multiwalled carbon nanotubes on the Thermal conductivity and cellular size of polyurethane foam. Advances in Polymer Technology, 1-8.
* 02 National puplications and 01 report in the conference proceeding
3. Kieu Thi Quynh Hoa and Nguyen Thi Yen (2023). Biosurfactant production by a crude oil-utilizing bacterium towards petroleum hydrocarbon biodegradation applications. Vietnam Journal of Chemistry. ISSN: 2572-8288, 1-7
4. Kieu Thi Quynh Hoa, Nguyen Vu Giang, Nguyen Thi Yên, Mai Duc Huynh, Nguyen Huu Dat, Vương Thi Nga, Nguyen Thi Thu Ha, Pham Thi Phuong (2020). Enhanced bioremediation of crude oil polluted water by a hydrocarbon-degrading bacillus strain immobilized on polyurethane foam (PUF). Journal of Biotechnology; 18(3): 581-588.
5. Kieu Thi Quynh Hoa, Vương Thi Nga, Nguyen Thi Yên, Nguyen Thi Thu Tra, Nguyen Thi Men, Nguyen Thi Diem Quynh, Nguyen Xuân Cảnh (2022). Study on effect of nitrogen on growth of yeast and bioremediation of petroleum-contaminated sand. Vietnam National Conference on Biotechnology 2022. 2022: 1365-1371.
- Patents (list):
1. Patent "Thermophilic and Halophilic Bacterial Strain, Bacillus flexus DSVK7, Capable of Synthesizing Biosurfactants and Degrading Petroleum Hydrocarbons. "Legal Acceptance Information: Accepted as a valid application under Decision No. 123287/QĐ-SHTT.ip dated December 25, 2023, issued by the Intellectual Property Office of Vietnam.
2. Patent "Method for Producing Polyurethane Foam immobilized petroleum hydrocarbon degrading microorganisms" Legal Acceptance Information: Accepted as a valid application under Decision No. 8117/QĐ-SHTT.ip dated January 24, 2024, issued by the Intellectual Property Office of Vietnam- Technological products (describe in details: technical characteristics, place):
- Specific products (product description, place of storage): 

Images of project
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