Project's information
| Project's title | Study on obtaining anti-cancer compounds from marine fungi isolated from sponges in Khanh Hoa sea area |
| Project’s code | VAST06.02/21-22 |
| Research hosting institution | Nha Trang Institute of Technology Research and Application |
| Project leader’s name | Dr. Phan Thi Hoai Trinh |
| Project duration | 01/01/2021 - 31/12/2023 |
| Project’s budget | 600 million VND |
| Classify | Excellent |
| Goal and objectives of the project | Evaluation of the biosynthetic ability of substances with anti-cancer activity of marine fungal strains isolated from sponges in Khanh Hoa sea area to obtain anti-cancer compounds with high activity for application in pharmacy. |
| Main results | - Theoretical results:
+ Isolation of 65 fungal strains from 36 sponge samples collected in Nha Trang Bay and screening of 30/65 fungal strains showing cytotoxic activity against MCF-7 and Hela cells.
+ Identification of 11 fungal strains with high cytotoxic activity, including 05 strains belonging to genus Aspergillus, 02 strains of genus Penicillium, 03 strains of genus Fusarium, and 01 strain of genus Trichoderma.
+ Determination of suitable fermentation conditions to obtain crude extract with high amount and anti-cancer activity from selected fungal strain Aspergillus sp. 1901NT-1.2.2: 22 days, sea salt concentration (30 g/L) and pH 8.0.
+ Isolation of 09 natural compounds including: vismione E (1) and endocrocin (2) from Aspergillus sp. 1901NT-1.2.2, lopouzanone A (3), lopouzanone B (4), gliorosein (5), balticolide (6), dendrodolide G (7), dendrodochol B (8) and 1-Oacetyl dendrodochol B (9 ) from L. pouzarii 168CLC-57.3, in which lopouzanone A (3), lopouzanone B (4) are new compounds.
+ Vismione E (1), endocrocin (2), gliorosein (5) and balticolid (6) showed cytotoxic activity against Hela, PC-3 and MCF-7 cancer cell lines.
+ Developed a fermentation process for the fungal strain Aspergillus sp. 1901NT-1.2.2 to obtain high amount of vismione E (1).
+ Developed a process to separate and obtain vismione E (1) and endocrocin (2) from the fungal strain Aspergillus sp. 1901NT-1.2.2.
- Applied results: The results showed that fungi isolated from sponges in Nha Trang Bay could be a potential source for further sutdy to discover natural compounds with cytotoxic activity against human cancer cell lines for application in medicine. |
| Novelty and actuality and scientific meaningfulness of the results | This is the first study to isolate vismione E from the marine fungal strain Aspergillus sp. 1901NT-1.2.2 and determine cytotoxic activity against MCF-7 and PC-3 cancer cell lines. Besides, the project also isolated two new natural compounds, lopouzanone A and B, from the marine fungal strain Lopadostoma pouzarii 168CLC-57.3. |
| Products of the project | - Scientific papers in referred journals (list):
1/ Phan Thi Hoai Trinh, Anton N. Yurchenko, Olga O. Khmel, Trang Vo Thi Dieu, Ngo Thi Duy Ngoc, Elena V. Girich, Alexander S. Menshov, Natalya Y. Kim, Ekaterina A. Chingizova, Tran Thi Thanh Van, Jong Seok Lee, Hyi-Seung Lee and Ekaterina A. Yurchenko (2022) Cytoprotective Polyketides from Sponge-Derived Fungus Lopadostoma pouzarii. Molecules, 27(21), 7650.
2/ Elena V. Girich, Phan Thi Hoai Trinh, Liliana E. Nesterenko, Roman S. Popov, Natalya Yu. Kim, Anton B. Rasin, Ekaterina S. Menchinskaya, Aleksandra S. Kuzmich, Ekaterina A. Chingizova, Artem S. Minin, Ngo Thi Duy Ngoc, Tran Thi Thanh Van, Ekaterina A. Yurchenko, Anton N. Yurchenko, and Dmitry V. Berdyshev (2023) Absolute Stereochemistry and Cytotoxic Effects of Vismione E from Marine Sponge-Derived Fungus Aspergillus sp. 1901NT-1.2.2. International Journal of Molecular Sciences, 24, 8150.
3/ Phan Thi Hoai Trinh, Ekaterina A. Yurchenko, Anton N. Yurchenko, Ngo Thi Duy Ngoc, Vo Thi Dieu Trang, Cao Thi Thuy Hang, Tran Thi Thanh Van, Pham Duc Thinh, Huynh Hoang Nhu Khanh, Le Dinh Hung, Nguyen Ho Cong Dung (2022) Evaluation of cytotoxic activity of marine fungi isolated from sponges in Nha Trang Bay. Vietnam Journal of Marine Science and Technology, 22(1), 51–57.
- Education: Trained 01 master
+ Name of graduate student: Nguyen Ho Cong Dung,
+ Master thesis title: Evaluation of the biosynthesis of anticancer agents of some fungal strains isolated from sponges in Khanh Hoa sea area.
+ Supervisor: Assoc. Prof. Dr. Tran Thi Thanh Van.
- Technological products (describe in details: technical characteristics, place):
+ A collection of 65 marine fungal strains isolated from sponges in Khanh Hoa sea area was stored at Nha Trang Institute of Research and Technology Application.
+ Data set on chemical structure and anticancer activity of 09 natural compounds isolated from selected fungal strains.
+ Profile of fermentation process of selected fungal strains for biosynthesis of secondary metabolites with high amount and anticancer activity.
+ Profile of obtaining process of 02 compounds with anticancer activity from selected strains of fungi. |
| Images of project |
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