Vietnam Academy of Science and Technology

Project's information

Project's title	Study on the composition and content of lipids, fatty acids and their derivatives from marine organisms in the South Central of Vietnam (Khanh Hoa-Binh Thuancoastal areas)
Project’s code	TĐDLB0.04/20-22
Research hosting institution	Institute of Natural Products Chemistry
Project leader’s name	Professor Pham Quoc Long
Project duration	01/01/2020 - 31/03/2024
Project’s budget	6,500 million VND
Classify	Grade B
Goal and objectives of the project	- Systematic survey on the composition and content oflipids, fatty acids and their derivatives from marine organisms (corals, seaweeds, echinoderms, mollusks, sponges) in the South Central of Vietnam (Khanh Hoa-Binh Thuan coastal area). - Create functional food products from marine organisms for practical applications.
Main results	- Theoretical results: + The project has collected 125 samples of marine organisms, including 34 samples of Coral, 21 samples of Mollusks, 19 samples of Seaweed, 32 samples of Echinoderm, 19 samples of Sponges in the coastal areaof Khanh Hoa - Binh Thuan. + Conduct lipid research on all collected samples:obtain data on total lipid content, composition and content of fatty acids in total lipids, composition and content of lipid classes in total lipids, composition and the content of fatty acids in the polar and non-polar lipid fractions of 30 samples;Analyze the molecular species of several classes of polar lipids in 5 marine samples. Conducted biological assays of all 125 total lipid extracts.Successfully built a database of lipid for 125 marine organisms. + The 1-O-alkyl-2-DHA-phosphatidylcholine mixture was prepared from tuna liver raw materials including 4 stages, using chromatography and synthesis techniques:Isolate PC and lyso PC from total lipid mixture using column chromatography,prepare 1-O-alkyl-2-OH-phosphatidylcholine mixture by hydrolysis reaction of PC + Lyso PC in alkaline environment, and prepare 1-O -alkyl-2-DHA-phosphatidylcholine by reaction of 1-O-alkyl-2-OH-phosphatidylcholine with DHA using DCC/DMAP catalyst. The products were analyzed by MS spectra. + Studied and evaluated the nutritional and chemical indicators of black seahorses raised in the South Central Coast (Khanh Hoa).From black seahorse raw materials, a technological process for hydrolyzing black seahorse has been built using the method of using ultrasound combined with enzymes to create a hydrolyzed seahorse powder product containing all the active ingredients. - Applied results: + Based on hydrolyzed seahorse powder,successfully prepared and produced the product namely: "Hải mã đan" to enhance health protection.The product has had its circulation declaration dossier prepared according to regulations at theVietnam Food Safety Authority. The product is expected to be approved for circulation after the project finish.
Novelty and actuality and scientific meaningfulness of the results	- Collect samples, study on lipids and biological activities of lipid extracts. build a lipid database for 125 marine organisms collected in the South Central of Vietnam (Khanh Hoa - Binh Thuan coastal areas). - Successfully researched the process of creating 01 product from black seahorse materials, successfully prepared and produced the product namely: "Hải mã đan" to enhance health protection. - Successfully researched the process of preparing phospholipid-based DHA mixture from marine organisms.
Products of the project	- Scientific papers in referred journals (list): + 03 International publication: 1. Thi-Kim-Hoa Dinh, Phi-Hung Nguyen, Doan Lan Phuong, Thi-Phuong-Ly Dang, Pham Minh Quan, Thi-Kim-Dung Dao, Valeria P. Grigorchuk and Pham Quoc Long (2023). Component and Content of Lipid Classes and Phospholipid Molecular Species of Eggs and Body of the Vietnamese Sea Urchin Tripneustes gratilla. Molecules, 28(9), 3721. (IF = 4,927; SCIE). 2. Pham Minh Quan, Hoang Ba Quang Anh, Nguyen Thi Nguyet Hang, Dao Huy Toan, Dao Viet Ha, Pham Quoc Long (2022). Marine derivatives prevent E6 protein of HPV: an in silico study for drug development. Regional Studies in Marine Science, 56, 102619. (IF = 2,166; SCIE). 3. Tatyana V. Sikorskaya, Ekaterina V. Ermolenko and Pham Quoc Long (2023). Betaine lipids of Symbiodiniaceae hosted by Indo‐Pacific corals. Phycological Research.(IF = 1,56; SCIE). - 02 Domestic publication: 4. Dang Thi Phuong Ly, Pham Minh Quan, Dang Thi Minh Tuyet, Ekaterina V. Ermolenko, Pham Quoc Long(2022). Molecular species of some glycerophospholipid classes of soft coral Sinularia leptoclados collected in Nha Trang, Khanh Hoa. Vietnam Journal of Marine Science and Technology, 22(4), 407-414. 5. Dao Thi Kim Dung, Pham Minh Quan, Pham Quoc Long, Nguyen Thi Nga, Dinh Thi Tu, Sikorskaya T. V., Ermolenko E. V., Dang Thi Phuong Ly, Nguyen Anh Hung (2022). Study on the year-round fluctuation of total lipid content and lipid classes composition in soft coral Sinularia flexibilis from Nha Trang, Khanh Hoa coastal. Vietnam Journal of Marine Science and Technology, 22(1), 59-65. - Patents (list): Title: Production process for hydrolyzed seahorse powder products Authors: Pham Quoc Long, Dang Thi Phuong Ly, Trinh Thi Thu Huong, Dang Thi Minh Tuyet, Dao Thi Kim Dung, Nguyen Thi Nga, Lai Phuong Phuong Thao, Nguyen Minh Vuong, Le Hong Nhung, Hoang Thi Bich Application number: 1-2024-00677 Accepted according to Decision No. 18121/QĐ-SHTT dated February 26th, 2024 of the National Office of Intellectual Property of Vietnam. - Technological products (describe in details: technical characteristics, place): + Specimens of 125 marine organisms collected in the South Central of Vietnam (Khanh Hoa - Binh Thuan coastal areas). All specimens are stored at Institute of Marine Enviroment and Resources. + Database on lipids of125 marine organisms collected in the South Central of Vietnam (Khanh Hoa - Binh Thuan coastal areas). (Web link: http://thqt.imas.vn:8087/index.php?). + Process for preparing phospholipid-based DHA mixture from marine organisms materials. + Process of creating 01 functional product from marine organisms at laboratory scale. + Product namely "Hải mã đan": quantity of 20,000 capsules (stored at the Institute of Natural Products Chemistry 1800capsules). - Other products (if applicable):Contributing to the training of 02 PhD students. + PhD student name: Tran Duy Phong Project name: Research on using some advanced techniques to process brown seaweed into useful products. Instructor 1: Professor Pham Quoc Long Instructor 2: Associate Professor Tran Quoc Toan + PhD student name: Dinh Thi Kim Hoa Project name: Research on the chemistry and biological activities of yellow sea urchins and black sea urchins in the coastal of Nha Trang, Khanh Hoa and application orientation in food technology. Instructor 1: Associate Professor Doan Lan Phuong Instructor 2: Dr. Nguyen Phi Hung
Recommendations	According to the obtained research results , the Principal Investigator commits to take responsibility for the accuracy of the above information and recommends continuing to implement the next phase in the Southern Sea region (Vung Tau – Ca Mau coastal areas) to complete the database on marine lipids in Vietnamsea.
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