Project's information

Project's title Structure and function of ribosome-inactivating proteins in development of new generation of recombinant toxins
Project’s code QTRU05.01/21-23
Research hosting institution Institute of Biotechnology
Coordinating unit, co-chair Eurasian Scientific Research Support Association
Project leader’s name PhD.Le Thi Bich Thao; Prof. Usanov Sergey Aleksandrovich; PhD Kozlov Sergey Aleksandrovich and PhD Oshchepkova Julia Igorevna
Project duration 01/01/2022 - 31/12/2023
Project’s budget 300 million VND
Classify Excellent
Goal and objectives of the project
The goal of the task is to study the relationship between structure and function of the ribosome-inactivating protein Trichobakin (TBK) to create a recombinant immunotoxin with a new sequence and structure with potential for effective application
Main results

The results achieved are (1) Designed, purified different forms of recombinant proteins ΔC7-TBK, ΔC7-TBK-GFP and evaluated anti-cancer activity on some human cancer cell lines; Identifying the ΔC7-TBK-GFP protein that has the ability to enter cells; (2) Designed and purified mutant molecules of Trichobakin labeled with different isotopes. Molecular structure simulation of forms of TBK fused to potential active peptides, antibody fragments, receptor ligands and their interactions with associated proteins; (3) Simulate and analyze the molecular structure of mutant forms of Trichobakin, their interaction with lipid bilayer membranes and with ribosomal proteins of Eukaryotic organisms; Evaluation of the inhibitory activity of extracellular protein biosynthesis and anti-cancer activity of mutant forms of Trichobakin
Publications: In vitro and in vivo anti-tumor effect of Trichobakin fused with urokinase-type plasminogen activator ATF-TBK. Molecular Biology Reports, 51:130 (2024)

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